RAPID ISOLATION OF SOYBEAN DNA FOR PCR WITH LARGE NUMBERS OF SMALL SAMPLES
Zhou Sijun (Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences,Harbin,150086)
The problem of grinding large numbers of small samples of fresh soybean tissues rapidly and without loss was solved by using a grinder modified with a spiral ratchet screw driver. The effect of different extraction buffers, different temperatures and different treatment times on the quality of DNA were compared by examining the DNA with random-primer PCR. The result showed that soybean templet DNA suitable for PCR reaction could be gotten by using SDS extraction buffer, 5 minutes treatment with 65-80℃ and one time of extraction with chloroform and iso-amyl alcohol. DNA was isolated from more than a thousand soybean samples with this procedure. The effect was stable.
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| China Proceedings of conference Full-text Database |
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ZHANG Ze-hua~1 LIU Meng-juan~2 ZHANG Min-juan~1 ZHAO Hui-xian~(1*) HU Sheng-wu~(2*) (1.College of life sciences,Northwest A & F University,Yangling,Shaanxi 712100,China; 2.College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China);A fast method of extracting DNA from the dry seeds of crops[A];[C];2007 |
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