Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Soybean Science》 2001-03
Add to Favorite Get Latest Update

INTRODUCTION OF Bt GENE(cryIA)INTO SOYBEAN BY Agrobacterium-MEDIATED TRANSFORMTION

Zhou Sijun 1 Li Xichen 1 Liu Zhaojun 1 Liu Liyan 1 Yang Qingkai 2 (1 Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 2 Institute of Soybean, Northeast Agricultural University, Harbin 150030)  
Bacillus thuringiensis cryIA gene was introduced into soybean successfully with Agrobacterium-cotyledonary node transformation system. Cotyledonary node explants were prepared from 5-7 day old seedlings, infected and co-cultivated with Agrobacterium tumefaciens. Resistant adventitious buds emerged after 4 weeks on selective medium. The buds were transferred onto elongation medium and regenerated shoots grew to 2.5-3 cm high after 4-6 weeks. The shoots were cut off and transferred onto rooting medium . After about 3 weeks, the rooted plantlets were hardened off and transferred into pots. All the plants could flower and set seeds normally. Among the 8 transplanted survival T 0 plants, seven gave positive PCR reaction; and 4 of the 7 T 1 plant lines had PCR positive plants. DNA was extracted from the leaves of positive plants in the 4 T 1 plant lines, and southern blot was conducted with probe of Bt gene. All the 4 lines are positive, indicating that the Bt gene has integrated into the receiver's genome and can be passed to progenies.
【CateGory Index】: S565.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 SUN Wen-li et al (College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110161);Advance in Research on the Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2008-16
2 Wang Ping Wang Gang Ji Jing Zhou Zhiming Gui Wei La Ba Wu Ying (Research Center For Plant Genetic Engineering, Changchun University of Agricultural and Animal Sciences, Changchun 130062);GENETIC TRANSFORMATION OF IMMATURE COTYLEDON VIA AGROBACTERIUM TUMEFACIENS IN SOYBEAN[J];Soybean Science;2004-02
3 Wang Xiaochun 1,2,3 Liu Shangqian 3 Ji Jing 1,2 Wang Gang 1,2 Wang Ping 2 (1.Tianjin University of Agricultural and Biological Engineering,Tian jin; 2.Changchun University of Agricultural and Animal Sciences, Changchun 130062; 3.Department of Agronomy,HeBei North University, Xuanhua 075131);OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROBACTERIUM TUMEFACIENS[J];Soybean Science;2004-03
4 Wang Xiaochun 1,2,3 Wang Gang12 Ji Jing12 Wang Ping2 Liu Shangqian3 (1.Tianjin University of Agricultural and Biological Engineering Academy,Tianjin 300072; 2.Huaihai Institute of Techndogy,Marine School,Lianyungang, 222005; 3.Department of Agronomy,Hebei North University, Xuanhua 075131);THE FACTORS INFLUENCING GENETIC TRANSFORMATION SYSTEM IN SOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROBACTERIUM[J];Soybean Science;2005-01
5 WANG Ping1,GAO Shi-qing2,GUO Yong-lai3,CHENG Xian-guo4,WANG Gang5,MA You-zhi2,and JI Jing5(1 School of Marine Technology and Aquiculture,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu ;2 Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081;3Changchun Longyang Soybean Biologic Albumen Co.Ltd,Changchun 130062,Jilin;4 Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;5 Agriculture and biology engineering college,Tianjin University,Tianjin 300072,China);Transformation of Stress Resistance Related Gene GmDREB into Soybean via Agrobacterium-mediation[J];Soybean Science;2008-01
6 WANG Quan-wei,ZHANG Hai-ling,BAI Jing,XU Xiang-ling(Life Science and Technology Institute,Harbin Normal University,Harbin 150025,Heilongjiang,China);Agrobacterium-Mediated Plant Transformation of Soybean[J];Soybean Science;2008-02
7 YUAN Cheng-zhi et al(College of Life Science and Engineering,Qiqihar University,Qiqihar,Heilongjiang 161006);Construction of Bt Gene-mediated Aphids-resistant Expression Vector in Soybean[J];Journal of Anhui Agricultural Sciences;2009-23
8 WANG Xiao-chun,LIU Shang-qian,LUO Yong-hua(Academy of Agriculture and Forest of Hebei North University,Xuanhua 075131,Hebei,China);Research Progress on Soybean Tissue Culture System and Transformation[J];Soybean Science;2009-04
9 SUN Wen-li1,2,3,LIU Yu-hui2,3,WU Yuan-hua1,FU Jun-fan1 (1.Plant Pathology Department, Shenyang Agriculture University, Shenyang 110161,China; 2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 3.Important Scientific Engineerings of Crops Gene Resources and Gene Improvement of China,Beijing 100081,China);A Pilot Study of An Soybeans Embryonic Tip Regeneration System[J];Hubei Agricultural Sciences;2008-06
10 WU Ying 1,WANG Ping 1,LIU Hai-xue 1,2,JI Jing 1 (1.Research Center for Plant Genetic Engineering,The Quartermaster University of PLA,Changchun 130062,China; 2.Agricultural College,Inner Mongolia University for Nationalities,Tongliao 028042,China);Agrobacterium- Mediated Tranformation in Soybean[J];Journal of Inner Mongolia University for Nationalities(Natural Sciences,Quarterly);2003-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Nan Xiangri Liu Wenping Liu Liyan Lu Xiaobo He Yunxia (Bio.Res. Center,Heilongjiang Academy of Agri.Sci. Harbin, 150086) Wei Zhiming (Shanghai Institute of Plant Physiology,Academia of Sinica, Shanghai, 200032);PEG-MEDIATED TRANSFORMATION AND REGENERATION OF SOYBEAN PROTOPLAST WITH BACILLUS THURINGIENSIS CrgIAc GENE[J];SOYBEAN SCIENCE;1998-04
2 ZHOU Si jun (Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China);The Major Obstacles and Research Progress on Soybean Transformation[J];Heilongjiang Agricultural Science;2001-03
3 NAN Xiang Ri (Biotechnology Research Center, Heilongjiang Academy of Agriculture Science,Harbin 150086) WEI Zhi Ming (Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, National Laboratory of Plant Molecular Genetics, Shanghai 200032);PROMOTION OF TRANSFORMATION FREQUENCY OF SOYBEAN (GLYCINE MAX L. ) PROTOPLASTS USING POLY-L-ORNITHINE[J];Acta Biologiae Experimentalis Sinica;1999-04
4 SU Yan_Hui 1WANG Hui_Li 2YU Mei_Min 1L De_Yang  3GUO San_Dui 1(Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101) 2(College of Life Sciences, Peking University, Beijing 100871);Studies on Transfer of Bt Gene into Glycine max[J];ACTA BOTANICA SINICA;1999-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Hai-yan et al (Changshu Institute of Technology, Changshu, Jiangsu 215500);Study on Secondary Metabolism Genetic Engineering about Insect Resistance[J];Journal of Anhui Agricultural Sciences;2005-10
2 SUN Wen-li et al (College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110161);Advance in Research on the Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2008-16
3 LI Tao et al(Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Branch of National Center for Soybean Improvement,Key Laboratory of Crop Genetics and Breeding,Shijiazhuang,Hebei 050031);Research Progress on Transgenic Technology of Soybean[J];Journal of Anhui Agricultural Sciences;2009-22
4 LI Gui-lan,QIAO Ya-ke,HAN Jin-ling,BI Yan-juan,ZHAO Shu-guang(Hebei Normal University of Science & Technology,Changli 066600,China);Influencing factors on the survival rate of transplanted plants from Agrobacterium-mediated transformation systems of soybean cotyledonary node[J];Journal of Agricultural University of Hebei;2007-02
5 ZHOU Si jun 1,LI Xi chen 1,LIU Zhao jun 1, LIU Li yan 1 and YANG Qing kai 2 (1 Biotechnology Research Center,Heilongjiang Academy of Agricultural Sciences,Harbin 150086; 2 Institute of Soybean,Northeast Agricultural University,Harbin 150030;Optimization of the Agrobacterium-mediated transformation systems of soybean[J];Journal of Northeast Agricultural University;2001-04
6 WU Xiao-xia,LI Wen-bin ( Soybean Research Institute, Northeast Agricultural University, Harbin Heilongj iang 150030, PRC );Research progress and development direction of soybean pest-resistance gene engineering[J];Journal of Northeast Agricultural University;2005-04
7 FENG Tao1,SUN Congshu1,LIU Lijun1,ZU Wei1,HAN Tianfu2(1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China; 2.Chinese Academy of Agricultural Sciences,Beijing 100081,China);Advances in the genetic transformation of soybean and its application in agriculture[J];Journal of Northeast Agricultural University;2008-03
8 LiuBeidong1 ZhuYanming2 YangQian1 YangXiaohui3 (1 Department of Life Science and Engineering ,HarbinInst .ofTechnology ,Harbin 15 0 0 0 1; 2 Life Science Collage ,Northeast Agriculture University ,15 0 0 30 ;3 Qiqihaer workS tation of Land );RECENT ADVANCES IN SOYBEAN TISSUE CULTURE AND GENE TRANSF OR MATION[J];Soybean Science;2003-01
9 Wang Xiaochun 1,2,3 Liu Shangqian 3 Ji Jing 1,2 Wang Gang 1,2 Wang Ping 2 (1.Tianjin University of Agricultural and Biological Engineering,Tian jin; 2.Changchun University of Agricultural and Animal Sciences, Changchun 130062; 3.Department of Agronomy,HeBei North University, Xuanhua 075131);OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROBACTERIUM TUMEFACIENS[J];Soybean Science;2004-03
10 Wu Xiaoxia Li Wenbin * * Zhang Shuzhen (Soybean Research Institute of Northeast Agriculture University,Harbin 150030);THE RESEARCH ADVANCE ON SOYBEAN TRANSGENE IN CHINA[J];Soybean Science;2005-02
China Proceedings of conference Full-text Database 1 Hits
1 Wang Pei-wu Wu Li-min Zhang Jun Yao Dan Chai Xiao-jie Guan Shu-yan (Biotechnology Center, Jilin Agricultural niversity, Changchun, Jilin 130118);A Study on Transformation of Bt + CpTI Insect Resistant Gene into Soybean[A];[C];2004
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HU Wen-ming et al(Institute of Plant Science and Technology,Tarim University,Alar,Xinjiang 843300);Study and Application of the Exogenous DNA Introduction Technique Mediated by Pollen Tube Pathway[J];Journal of Anhui Agricultural Sciences;2007-36
2 Li Lanfang, Wu Shuxun, Zhang Kui, Zhang Jie (Hebei Academy of Medical Sciences, Shijiahuang 050021);Content of Free Amino Acids in Bothriochloa ischaemum in Different Growing Phase[J];AMINO ACIDS & BIOTIC RESOURCES;1997-03
3 ZHAN Qiuwen 1,2 ,CAI Junyi 1 (Soybean Research Institute,Nanjing Agricultural University;National Center of Soybean Improvement,Nanjing 210095; 1,2 Anhui Agrotechnical Teachers College,Fengyang 233100);Genotypic Variation and Germplasm Evaluation of Soybeans in Resistance to Leaf-feeding Insects[J];JOURNAL OF ANHUI AGROTECHNICAL;1999-04
4 n WEI Ge ZHANG Beilin TANG Jianguo HU Meihao(College of Life Sciences,Peking University,Beijing,100871);Construction and Expression of the Gene for“Mini-C”Analog of Human Proinsuli[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1994-06
5 LI Xiongbiao XIE Bin CHEN Laitong TANG Jianguo HU Meihao(College of Life Sciences,Peking University,Beijing, 100871);Construction and Expression of the Gene for Human Proinsulin Analog(B-Arg-Arg-A)[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1995-01
6 Gao Yike; Zhao Bo; Ding Guoxun; Zhang Qixiang. Journal of Beijing Forestry University (2001)23(1)32~33[Ch,7 ref.]Department of Landscape Architecture, Beijing For. Univ., 100083, P.R.China.;Shoot regeneration from stem and leaf explant of Dendrathema grandiflorum.[J];Journal of Beijing Forestry University;2001-01
7 Jin Wenlin et al (Beijing Agricultural College, Beijing 102206);The Study of Adzuki Bean Variety Sources of North China VI The Observing of the Leaf Blade Hair Character of Adzuki Bean[J];JOURNAL OF BEIJING AGRICULTURAL COLLEGE;2000-03
8 XU Hui,ZHAO Li-ping Department of Life Science,Binzhou University ,Binzhou 256603,China;Mechanisms and Appraisal of Plant Resistance to Salt Stress[J];Journal of Binzhou Teachers College;2005-03
9 YUAN Zhi-dong,YAO Hong-wei,YE Gong-yin,HU Cui(Institute of Insect Sciences /State Key Laboratory of Rice Biology,Zhejiang University,Hangzhou 310029,China);Survival Analysis of the Larvae from Different Hybrids of Silkworm,Bombyx mori Exposure to Bt Rice Pollen[J];Bulletin of Sericulture;2006-03
10 Bai Shuxia; Liu Liqiu; Chen Wenlong(Institute of Agricultural Medernization, CAS, Shijiazhuang 050021 );Review on High Quality Somatic Embryogenesis in Artificial Seed of Plant[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 SUN Wen-li et al (College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110161);Advance in Research on the Regeneration System of Soybean[J];Journal of Anhui Agricultural Sciences;2008-16
2 LI Tao et al(Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Branch of National Center for Soybean Improvement,Key Laboratory of Crop Genetics and Breeding,Shijiazhuang,Hebei 050031);Research Progress on Transgenic Technology of Soybean[J];Journal of Anhui Agricultural Sciences;2009-22
3 WU Xiao-xia,LI Wen-bin ( Soybean Research Institute, Northeast Agricultural University, Harbin Heilongj iang 150030, PRC );Research progress and development direction of soybean pest-resistance gene engineering[J];Journal of Northeast Agricultural University;2005-04
4 Zhang Shuzhen 1 Xu Pengfei 1 Lin Shifeng 1 Li Wenbin 1 * Yang Chuanping 2 * (1.Soybean Institute of Northeast Agricultural University,Harbin 150030; 2.Forestry College of Northeast Forestry University,Harbin 150040);RECENT ADVANCES AND PROSPECT ON SOYBEAN SOMATIC EMBRYOGENESIS SYSTEM[J];Soybean Science;2004-03
5 Luo Zhenfeng Li Qiyun (Northeast Agricultural University, Haerbin 150030;Jilin Academy of Agricultural Sciences, Gongzhuling 136100);COMMERCIALIZATION STATUS AND RESEARCH PROGRESS OF TRANSGENIC SOYBEAN[J];Soybean Science;2005-03
6 Cai Yirong Li Wangfeng Liu Lixia Xu Shoumin (School of Life Sciences,Northeast Normal University,Changchun 130024);GENETIC ENGINEERING ON IMPROVING SOYBEAN QUALITY TRAITS IN BREEDING[J];Soybean Science;2006-01
7 Zhang Yinxia1,2 Song Xiaohua1 Li Yinhui2 Qiu Lijuan2(1.College of Agronomy,Ningxia University,Ningxia750021;2.Institute of Crop Germplasm Resources,CAAS,Beijing10081);ADVANCES ON THE STUDY OF AGROBACTERIU M-MEDIATED TRANSFOR MATION OF SOYBEAN[J];Soybean Science;2006-03
8 JI Yue-mei1,2,CHEN Shou-yi3,LI Ying-hui2,ZHANG Li-juan2,SONG Xiao-hua1,CHANG Ru-zhen2,and QIU Li-juan2(1Ningxia University Agricultural College,Yinchuan 750021,Ningxia;2The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) / Key Laboratory of Germplasm & Biotechnology (MOA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,100081 Beijing;3The National Key Lab of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China);Optimization of Genetic Transformation System from Soybean Cotyledon Mediated by Agrobacterium[J];Soybean Science;2008-01
9 LIAN Yun,LIANG Hui-zhen,WANG Shu-feng,YU Yong-liang,WANG Ting-feng,WEI Yan-li(Institute of Economic Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China);Advances on Agrobacterium-mediated Genetic Transformation of Soybean and Present Status of Genetically Modified Crops[J];Soybean Science;2009-03
10 WANG Fei1,XING Xin-sheng3,MA You-zhi2,CHEN Yao-feng1,CAO Xin-you1 (1 College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;2 Institute of Crop Science,The Chinese Academy of Agricultural Sciences,Beijing 100081,China;3 Luntai Agricultural Technology Extension Center,Luntai,Xinjiang 841600,China);Study on the Conditions of Transferring D32 Gene into Tobacco Mediated by Agrobacterium tumefaciens[J];Acta Botanica Boreali-Occidentalia Sinica;2009-06
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Lei Bojun Yin Guangchu Lu Caihua Qian Hua Zhang Kaiwang Zhou Sijun Wang Shulin(Soybean Research Institute HAAS Harbin China);STUDY OF EXOGENOUS DNA DIRECTLY TRANSFERED INTO SOYBEAN[J];Soybean Science;1991-01
2 Xiao Wenyan Wang Lianzheng (Chinese Academy at Agticultural Sciences, Beijing, 100081);PLANTLET REGENERATION FROM PROTOPLASTS OF SOYBEAN(GLYCINE MAX L. )THROUGH EMBRYOGENESIS[J];Soybean Science;1993-03
3 Nan Xiangri Liu Wenping Liu Liyan Lu Xiaobo He Yunxia (Bio.Res. Center,Heilongjiang Academy of Agri.Sci. Harbin, 150086) Wei Zhiming (Shanghai Institute of Plant Physiology,Academia of Sinica, Shanghai, 200032);PEG-MEDIATED TRANSFORMATION AND REGENERATION OF SOYBEAN PROTOPLAST WITH BACILLUS THURINGIENSIS CrgIAc GENE[J];SOYBEAN SCIENCE;1998-04
4 NAN Xiang Ri (Biotechnology Research Center, Heilongjiang Academy of Agriculture Science,Harbin 150086) WEI Zhi Ming (Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, National Laboratory of Plant Molecular Genetics, Shanghai 200032);PROMOTION OF TRANSFORMATION FREQUENCY OF SOYBEAN (GLYCINE MAX L. ) PROTOPLASTS USING POLY-L-ORNITHINE[J];Acta Biologiae Experimentalis Sinica;1999-04
5 Zhou Si-jun,Yin Guang-chu,Lei Bo-jun,Lu Cui-hua,Zhang Kai-wang,Qian Hua (Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086);STUDIES ON THE INFLUENTIAL FACTORS OF THE SOMATIC EMBRYOGENESIS OF SOYBEAN[J];Chinese Bulletin of Botany;1992-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved