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《Soybean Science》 2001-03
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Ding Derong Gai Junyi (Soybean Research Institute, Nanjing Agricultural University; National Center of Soybean Improvement, Ministry of Agriculture, Nanjing 210095)  
Comparative observation on microspore development in cytoplasmic-nuclear male sterile soybean material, such as (N8855×N2899)F 1, BC 1 male sterile plants, NJCMS1A and its fertile countpart (N2899×N8855)F 1, maintainer line, was made with light and transmission electron microscopy. The result showed the abortion appearance of the different generations with the same cytoplasm was similar, pollen abortion occurred majorly from late mononucleate stage to binucleate stage. The abnormal vacuoles in microspore in (N8855×N2899)F 1 male sterile anther occurred at late mononucleate microspore stage. Plasmodium shriveled at mature pollen grain, The partial cytoplasm disintergrated, and 2-3 nucleus were found in some microspores in BC 1 sterile plants. Pollen abortion of NJCMS1A occurred at the stage of binucleate pollen. There appeared more small vacuoles in microspore cytoplasm of NJCMS1A than that of its maintainer line at the stage that generative cell closed to vegetative cell, starch grains not being produced in cytoplasm. After generative cell was away from vegetative cell, part of cytoplasm disordered, generative and vegetative nuclei further degenerated, vacuoles expanded, part of cytoplasm disintegrated, and the development of endexine was completely inhibited. Isozyme analysis indicated that the fastest cytochrome oxidase active band missed at binucleate stage, which was consistent with the result of the pollen abortion phase. Thus, the mechanism of the cytoplasmic-nuclear male sterility was probably associated with cytochrome oxidase.
【Fund】: 国家自然科学基金 ( 9390 0 13);; 国家攻关项目 ( 96 -0 0 2 -0 2 -12 -4 )
【CateGory Index】: Q943
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