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《Soybean Science》 2003-02
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THE SALT TOLERANCE OF CALLUS OF WILD/CULTIVATED SOYBEAN AND MDA CONTENT OF PLANT GROWN IN SALTY SOIL

Qiao Yake Li Guilan Wang Wenpo Gao Shuguo Bi Yanjuan Jing Yan (Hebei Vocation-Technical Teachers College , Changli, 066600)  
The 14 wild soybeans and cultivated soybeans were studied in this experiment. Hypocotyls were used as explants. Callus was induced at MS+2,4-D2.0mg/L+6-BA0.5mg/L + sucrose 3% + agar 0.8% . The salt tolerance of callus was observed on different medium containing 0(as contrast),0.1%, 0.2% ,0.3%, 0.4% ,0.5%,0.6%,0.7%,0.8% NaCl. The experimental materials were planted also in soil pond containing 0.0% ?0.3%NaCl. The results showed that salt tolerance of callus from Zhonghuang 4,Tongnong5 and Jilin35 was stronger. Among different materials, the difference of salt tolerance was remarkable. The higher salt tolerance materials could grow well on medium containing 0.8%NaCl , the lower salt tolerance materials could only grow on medium containing 0.4%NaCl. Suitable concentration for identifing salt tolerance of soybean callus was 0 6% NaCl in this experiment MDA content of plants in 0.3% NaCl was higher than that in control. MDA content of plants grown on 0.4% NaCl soil pond at different time appeared different varies with the increase of salt stress days.The correlation between MDA content and callus salt tolerance was not found in this paper.
【Fund】: 河北省科技厅项目 ( 992 30 313)。
【CateGory Index】: S565.1
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