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《Soybean Science》 2004-02
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EFFECTS OF DIFFERENT NITROGENS ON ACTIVITIES OF NITRATE REDUCTASE,GLUTAMINE SYNTHETASE AND SEED PROTEIN VONTENTS IN SOYBEAN VULTIVARS

Chen Yu 1,2 Zhu Baoge 1** Zhang Jing 1 Liang Zongsuo 2 (1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Bejing,100101; 2.College of Life Sciences, Northwestern Sci-Tech University of Agriculture and Forestry,Yangling,712100)  
Effects of different nitrogens(NO 3 --N,NH 4 +-N,NO 3 --NH 4 +)on nitrate reductase (NR) and glutamine synthetase(GS) activities, and seed protein contents of soybean cultivars during pod-bearing period were investigated. The experimental results indicated that NR activities of leaves in four soybean genotypes treated by NO 3 --N were obviously enhanced. However, NR activities of these genotypes treated by NH 4 +-N were the lowest in three kinds of nitrogens. GS activities induced by NO 3 --NH 4 + and NH 4 +-N were remarkably increased. Seed protein contents of four genotypes induced by three kinds of nitrogens were higher than those done by H 2O(CK). The correlation between leaf NR activity and seed protein content was extremely significant (r= 0.633 *). Hence it is suggested that high NR activity in soybean leaves should be one of bio-chemical index for selecting soybean germplasms with seed high protein.
【Fund】: 国家“8 63”计划项目 ( 2 0 0 1AA2 410 61-4 ;2 0 0 2AA2 0 70 0 7;2 0 0 2AA2 110 5 1) ;; “973”项目 ( 2 0 0 2CB1113 0 1);; 中国科学院知识创新项目 (KSCX2 -1-0 1-10 )的部分资助
【CateGory Index】: S565.1
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