ANALYSIS OF RNA LATION FROM DIFFERENT SOYBEAN SRGAS
Fu Chang Wang Yu-Ying Dai Hong-Jie (Life and Environment College, Harbin Normal University, Harbin, 150080)
Isolation of high-quality RNA is the base of studies on gene cloning and regulations on gene expression. Because of the difference in contents of components, the difficulties of isolating RNA differ from different plants or different organs of the same plant. We applied two methods of guanidine thiocyanat and an improved method by Wang Fang to find a suitable method for RNA isolation from embryo, leaf,stem and root of soybean. The results suggest that the method of guanidine thiocyanat fits for extracting RNA from soybean root and stem, and the improved method by Wang Fang fits for embryo as well as root and stem. Both of the two methods don't work very well for RNA isolation from soybean leafs.