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《Soybean Science》 2007-01
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OPTIMIZATION OF SSR DETECTION SYSTEM IN SOYBEAN

SONG Xian-jun,XIE Fu-ti,ZHANG Wei,CAO Ping,GUO Yu-hua(College of Agronomy,Shenyang Agricultural University,Shenyang 110161)  
Effects of major components of PCR amplification on soybean SSR detection results were studied.A SSR detection system suitable for soybean was established with improved PCR reaction system,PCR program and silver staining method.The optimized PCR amplification system was 10×Buffer 1.00μL,2.00 ng/μL template DNA,0.15 μmol/L SSR primer,150.00μmol/L dNTPs,0.50 U Taq enzyme,2.00 mmoL/L Mg2+,adding ddH2O to terminal volume 10.00 μL.The optimized PCR cycles started at 94℃(3min),followed by 35 cycles of 25s at 94℃,25s at 47℃ and 30s at 72℃,and then extended 5min at 72℃.The optimized soybean SSR detection system was proved more economic and useful.
【Fund】: 辽宁省教育厅科技项目(05L3781);; 辽宁省科学技术计划项目(2006201008)的资助
【CateGory Index】: S565.1
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