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MAPPING AND VERIFICATION OF GENES RELATED TO LINOLENIC ACID CONTENT IN SOYBEAN

LIU Li-jun,XUE Yong-guo,ZHAO Gui-xing,ZHANG Lei(Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086)  
Oil composition is one of critical factors affecting the qualities and processing properties of soybean,and linolenic acid content is closely related to the processing cost and qualities of soybean oil.In the experiment,Ha 89016 and Heihe 18 were used as parents to develop F2 generations,and bulked segregant analysis(BSA)method was adopted to detect SSR markers conferring linolenic acid content.Satt286 was found to closely link with genes controlling linolenic acid content,with a genetic distance of 9.5 cM.The average content of lower and higher linolenic acid individuals differentiated by Satt286 was 3.91% and 6.36%,respectively,and their difference reached 0.01 significant level.From the filial 4 generation of hybridization between H89016 and Heihe 18,13 individuals with lower linolenic acid content and 9 with higher linolenic acid content were selected and the accuracy between molecular marker and phenotypic value of each individual reached 82%,which confirmed the primer Satt286 closely linked with genes controlling linolenic acid content and could be used to select individuals differed in linolenic acid content.The expression level of gene Fad3c,which was related to linolenic acid content,were analyzed by real-time quantitative PCR method between soybean Ha 89016(lower linolenic acid content)and Heihe 18(higher linolenic acid content),and results showed the transcription rate of Ha-89016 was significantly lower than that of Heihe18,which further confirmed that the expression level of the Fad3c gene was related to the final linolenic acid content in different soybeans.
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