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《Soybean Science》 2008-02
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Inheritance and Gene Tagging of Male Fertility Restoration of Cytoplasmic-Nuclear Male Sterile Line NJCMS2A in Soybean

DONG Jian-sheng,YANG Shou-ping,YU De-yue,GAI Jun-yi(Nanjing Agricultural University,Soybean Research Institute,National Center for Soybean Improvement,National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,Jiangsu,China)  
Cytoplasmic-nuclear male sterility plays an important role in the production of hybrid seeds.The knowledge of inheritance of male fertility restoration of a cytoplasmic-nuclear male-sterile line is essential to hybrid seed production.With the rapid development of molecular markers,mapping male fertility restoration genes appears an effective way to study inheritance of the trait.The soybean cytoplasmic-nuclear male-sterile line NJCMS2A was developed through consecutive backcross procedures with the F2 male sterile plants of the N8855(N1628 cross as donor parent and N1628(designated as NJCMS2B afterwards)as recurrent parent.In the present paper,the inheritance of the male fertility restoration of NJCMS2A was studied,at the same time,the SSR(simple sequence repeat)marker linked to the restorer gene of NJCMS2A was screened.The results showed that the inheritance of the fertility restoration of NJCMS2A was steady among different years.All F1 plants of the NJCMS2A×Zhongdou 5 cross were male-fertile.The F1∶2 population exhibited male fertility segregation,and the segretation ratio of male-fertile to male-sterile plants fitted a 15∶1 ratio under χ2 test.The F1 and F1∶2 results proposed a possible inheritance model of two duplicate dominant genes.The segregation ratio of number of non-segregating male-fertile F2∶3 families∶number of 3∶1 segregation F2∶3 families∶number of 15∶1 segregation F2∶3 families fitted a 7∶4∶4 ratio by χ2 test as expected.All the above phenotypic and genotypic segregation data demonstrated the hypothesis that male fertility restoration of NJCMS2A was controlled by two pairs of duplicate dominant genes,which further verified the results of Bai and Gai.One F1:2 population from the NJCMS2A×Zhongdou 5 cross was chosen randomly to tag the restorer gene of NJCMS2A with 903 pairs of SSR primers selected randomly from the soybean public genetic linkage map reported by Cregan et al.The result showed that one SSR marker,e.g.Satt135 was linked to the restorer gene of NJCMS2A.The recombination ratio of the restorer gene of NJCMS2A with Satt135 calculated from the maximum likelihood method was 0.1127,and converted into Kosambi's genetic distance,was 11.47cM.In comparison with the integrated linkage map by Song et al.,the restorer gene of NJCMS2A was located on the linkage group D2.
【Fund】: 国家自然科学基金资助项目(30490250 30671314);; 国家重点基础研究发展计划资助项目(2002CB111304 2004CB117206 2006CB101708);; 国家高技术研究发展计划资助项目(2002AA211052 2006AA100104 2006AA10Z1C1);; 长江学者和创新团队发展计划资助项目(PCSIRT)
【CateGory Index】: S565.1
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