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《Soybean Science》 2008-02
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QTL Tagging for High Protein Gene and Using Molecular Marker Assistant Selection in Soybean Breeding

YANG Zhe,LIU Li-jun,GAO Ming-jie,PU Guo-feng,ZHANG Lei(Soybean Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China)  
Soybean protein content is a quantitative trait controlled by multi-genes,and tagging these genes by using molecular markers has strong applied values.F2 population made from Heinong 35×Heinong 45 were used to map high protein genes.251 pair of SSR primers covering the whole soybean genome were screened,and 40 pair of them were polymorphic between the parents.The genetic linkage map was constructed with Mapmaker Exp3.0 and QTL analysis was done by using Mapmaker QTL1.1.One high protein QTL linking with satt532 in the LG D1a+Q of soybean consensus map was tagged,the genetic distance was 0.2 cM,the variance ratio was 32.7%.The primer satt532 was used to exam and screen the high protein soybean materials,47 shares were screened from 56 ones,and the ratio of the examination reached 83.93%.The result suggests that the primer of satt532 has the common identity and can be used to screen the high protein soybeans.
【Fund】: 黑龙江省农业科学院科研计划资助项目
【CateGory Index】: S565.1
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