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《Soybean Science》 2008-05
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Extraction of Soybean Oligosaccharides by Microwave and Ultrasonic Technique

LIU Li-yang1,JIN Long-guo 2,LIU Zhang-Xiong 2,HAO Zai-bin 1,CHANG Ru-zhen2,QIU Li-juan 2(1 Life Science College of Northeast Agricultural University,Harbin 150030,Heilongjiang;2 The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Germplasm & Biotechnology(MOA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,100081 Beijing,China)  
To Investigate the extraction efficiency of soybean oligosaccharides by microwave and ultrasonic and provide experimental evidence for further soybean oligosaccharides extraction.The orthogonal experimental design is used to compare the extraction efficicency of microwave and ultrasonic extraction.The microwave extraction selected orthogonal experimental table L9(34) and the optimization condition was 70%ethanol,the ratio of material to solvent 1∶10,high power,2 minutes.The ultrasonic extraction selected orthogonal experimental table L16(44) and the optimization condition is 75%ethanol,the ratio of material to solvent 1∶10,40 KHz,60℃,1.5 hours.HPLC was used to measure the extraction efficiency.The optimization condition selected by standard substance stachyose,raffinose and sucrose were mobile phase acetonitrile∶water(60∶40),temperature 35℃,velocity of flow 1.0 mL·min-1.The results indicate the efficiency and effect of extraction by microwave are both better than that of extraction by ultrasonicand the microwave extraction method is a simple,easy-to-use and suitable method for soybean oligosaccharides extraction.
【Fund】: 国家“十一五”科技支撑计划资助项目(2006BAD13B05);; 国家高技术研究发展计划(863计划)资助项目(2006AA10A110 2006AA100104)
【CateGory Index】: S565.1
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