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Fast Analysis on Fatty Acids of Soybean Seed by Gas Chromatography

ZHANG Ying-jun1,GAO Hui-min2,JIANG Chun-zhi1,HU Meng-yun1,LIU Bing-qiang,LI Hui1(1 Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Key Laboratory of Crop Genetics and Breeding of Hebei Province,National Soybean Improvement Center Shijiazhuang Sub-center,Shijiazhuang 050031;2Cash and Crops Institute of Agriculture,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,Hebei,China)  
The content of fatty acids is an important index for oil crops.The traditional methods of detecting fatty acids need more samples and more time,which unsuitable to select hundreds of seeds for breeders.In this experiment,0.040 g soybean seed(about quarter of the soybean seed) was used to optimize the method of oil extraction and methyl esterification,and extracted oil from the soybean powder using the mixture(benzene:petroleum ether=1∶1) for 5 minutes,methyl esterificated using sodium hydroxide-methanol solution(c= 0.5 mol·L-1) for 7 minutes,then the supernatant fluid was injected into the gas chromatography system.Using this method,the work which usually need almost ten hours,now can be completed in 12 minutes,thus lots of time can be saved.By comparing with traditional method(GB/T 5009.6-2003,GB/T 17376-1998) for detecting fatty acid compositions,it was confirmed that the results got from this method was exact and credible.
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