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《Soybean Science》 2009-03
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Analysis of SSR Information in EST Resource of Soybean(Glycine max)

CHEN Xiang-yan1,LI Wei2,DAI Hai-ying2,ZHANG Li-feng2(1Office,Shandong Academy of Agricultural Sciences,Jinan 250100,Shangdong;2Crop Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China)  
Expressed sequence tags(ESTs)are important resources for development of new SSR markers.In this study,394 370 ESTs of soybean(Glycine max L.)in the database of NCBI were downloaded and analyzed.After the preprocession,resulting in 80 735 non-redundant ESTs with total length about 51 332.21 kb.Totally 7 754 SSRs distributed in 6 674 ESTs were detected,accounting for 8.27% of the non-redundant ESTs.The average length and distribution distance of the EST-SSRs were about 15.26 bp and 6.62 kb,respectively.Dinucleotide and trinucleotide repeats with similar frequency are the main types,accounting for 81.06% of all the SSRs.AG/CT and AAG/CTT are the most frequent motifs,accounting for 62.83% and 25.22% in the dinucleotide and trinucleotide repeats,respectively.These EST-SSRs will help to develop new SSR markers with high polymorphism for soybean.
【Fund】: 山东省自然科学基金资助项目(Y2006D20)
【CateGory Index】: S565.1
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