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《Soybean Science》 2010-01
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Effect of Soybean Hypocotyls on Differentiation of Colon Carcinoma Cell HT-29 in vitro

QUAN Ji-shu1,XU Hui-xian2,LI Tian1,YIN Xue-zhe2(1.Basic Medical College of Yanbian University,Yanji 133000,Jilin;2.Affiliated Hospital of Yanbian University,Yanji 133000,Jilin,China)  
Soybean isoflavones and saponins have been observed to induce the differentiation of various tumor cells.The effects of soybean hypocotyl extract(SHE),rich in isoflavones and saponins,on the proliferation and differentiation of colon carcinoma HT-29 cells were investigated.The immunocytochemical method was used for the determination of Ki-67 and PCNA,the spectrometric method was used for the detection of alkaline phosphatase(ALP)and lactate dehydrogenase(LDH)activities,the ELISA method was used for the assay of CEA.Results showed that SHE reduced the expression of Ki-67 and PCNA,and increased the ALP activity of colon carcinoma cells in a time-and concentration-dependent manner.However,no significant treatment effect was found for CEA and LDH levels.It is suggested that soybean hypocotyl,rich in isoflavones and saponins,could exert an anti-colon cancer effect via inhibition of cell proliferation and induction of cell differentiation.
【Fund】: 吉林省科技发展计划资助项目(200705428);; 国家自然科学基金资助项目(30360113)
【CateGory Index】: R735.3
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