Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Soybean Science》 2012-01
Add to Favorite Get Latest Update

Genetic Stability Analysis of the Fifth Generation of Transgenic Soybeans Expressing γ-linolenic Acid

CHEN Sheng1,GUO Li-qiong1,2,SONG Jing-shen1,LIN Jun-fang1,2(1.College of Food Science,South China Agricultural University;2.Institute of Biomass,South China Agricultural University,Guangzhou 510642,Guangdong,China)  
The genetic stability of foreign gene and separation of △6-fad and bar gene were observed in the 5th generation of transgenic soybeans expressing γ-linolenic acid.By PCR amplification and resistance testing of selective marker gene,the genome of transgenic soybeans TS824 and TS825 were integrated △6-fad gene(marker free);TS81,TS83,TS85,TS87,TS814,TS816 and TS818 were integrated bar gene;TS88,TS89,TS810,TS813,TS815,TS816,TS817,TS819 and TS820 were integrated both △6-fad gene and bar gene;neither △6-fad gene nor bar gene was detected in TS82 and TS86.The results showed that △6-fad gene was stably maintained in the genome of part of transgenic soybeans,and △6-fad and bar gene of transgenic soybeans TS824 and TS825 were separated.
【Fund】: 国家转基因生物新品种培育重大专项课题(2009ZX0004-007B);; 广东省科技攻关项目(2005B20101009 2009A020102004)
【CateGory Index】: S565.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 YAN Miao-miao et al(Yantai Campus of Binzhou Medical College,Yantai,Shandong 264003);A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method[J];Journal of Anhui Agricultural Sciences;2008-20
2 Zhang Xiuchun~1 Gou Liqiong~(1,2 *) Wu Kunxin~1 Lin Junyang~3 Lin Junfang~(1,2)(1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of TropicalAgricultural Sciences.Haikou 571101;2.Department of Bioengineering,College of FoodSciences,South China Agricultural University.Guangzhou 510640;3.Putian City'sInstitute of Agricultural Sciences.Putian,351100);AGROBACTERIUM-MEDIATED TRANSFORATION OF SOYBEAN WITH THE EXPRESSION VECTOR CARRYING TWO SEPARATE T-DNAs[J];Soybean Science;2005-04
3 Zhang Xiuchun~1 Peng Ming~1 Wu Kunxin~1 Guo Liqiong~(1,2) Lin Junyang Lin Junfang~(1,2) (1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101;2 Department of Bioengineering,College of Food Sciences,South China Agricultural University,Guangzhou 510640;3 Putian City's Institute of Agricultural Sciences,Putian 351100);GENERATING MARKER-FREE TRANSGENIC SOYBEAN PLANTS BY AGROBACTERIUM-MEDIATED TRANSFORMATION WITH DOUBLE T-DNA BINARY VECTOR[J];Soybean Science;2006-04
4 WEI Bing-qiang1,ZHAO Chang-zeng1,LU Lu2,WANG Fu-lan1,LI Wei-min1(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2.College of Life Science,Northwest Normal Univesity,Lanzhou 730070,China);Construction of the a-ACO1 gene of sweet melon expression vector with marker-free and its co-transformation to tobacco[J];Journal of Gansu Agricultural University;2007-05
5 JIAN Yuyu1, CHEN Yuanling1, LI Jing1, GAO Lili1, DONG Chun2 (1 College of Biotechnology, South China Agric.Univ.,Guangzhou 510642, China; 2 College of Resources & Environmental Sciences, South China Agric. Univ., Guangzhou 510642;Primary Studies on Inheritance and Expression of Foreign Gene in Transgenic Rice[J];Journal of South China Agricultural University;2001-01
6 ZHAO Peng, YAO Si-yu, LIU Rong-zhen, et al. (Guangxi Zhuang Autonomous region Center for Disease Control and Prevention, Nanning 530021, Guangxi, P. R. China);Experimental study on the effect of γ-linolenic acid in reducing blood lipids in animals.[J];China Tropical Medicine;2004-05
7 Zhang Xiuchun1 Lin Junfang1,2 Guo Liqiong1,2(1 National Key Laboratory for Tropical Crops Biotechnology,CATAS,Haikou,Hainan 571101) 2 Department of Biotechnology, College of Food Sciences,South China Agricultural University, Guangzhou,Guangdong 510640);Cloning of Δ~6-Desaturase Gene and Construction of Its Co-transformation Expression Vector[J];Chinese Journal of Tropical Crops;2004-04
8 TAN Deng-feng, HAN Zhao-xue, Cao Mo-ju, Pan Guang-tang, Rong Ting-zhao (Maize Research Institute, Sichuan Agricultural University, Yaan 625014, Sichuan, China);Co-Transformating Maize with Double T-DNA Vector of DREB Gene[J];Journal of Sichuan Agricultural University;2008-01
9 WANG Qing lian 1,ZHANG Bao hong 2,GUO Teng long 3,XU Xin ran 1 (1.Henan Vocation Technical Teachers College,Xinxiang 453003,Henan,China; 2.Key Laboratory of Cotton Genetic Improvement of the Ministry of Agriculture,Cotton Research Institute;lnheritance of Exogenous Genes in Transgenic Cotton[J];Life Science Research;2001-04
10 ZHANG Xin Mei 1,2 , XU Hui Jun 1,* , DU Li Pu 1, YE Xing Guo 1, XIN Zhi Yong 1, GUO Ai Guang 2, XUE Song 2, MA You Zhi 1 ( 1 Institute of Crop Breeding and Cultivation CAAS, Ministry Key Lab for Crop Genetics and Breeding, Beijing 100081; 2 College of Life Science, Northwest Sci Tech University of Agriculture and Forestry, Yangling, Shaanxi, 712100, China);Excision of bar Gene from Transgenic Wheat Obtained by Biolistic Co-transformation[J];Acta Agronomica Sinica;2004-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jie et al(The College of Life Science,Agriculture University of Hebei,Baoding,Hebei 071001);Progress in Marker-free Transgenic Plants[J];Journal of Anhui Agricultural Sciences;2009-12
2 HUANG Sheng-he1,DING Yong-hong1,HASI Agula2(1.Fuzhou Medical College of Nanchang University,Fuzhou,Jiangxi 344000;2.College of Life Sciences,Inner Mongolia University,Huhhot,Inner Mongolia 010021);Research Progress on Δ~6-Fatty Acid Desaturases of Plant[J];Northern Horticulture;2011-01
3 TAN Jiang-ping~1,ZENG Xiu-li~(2,3),LIAO Ming-an~1,QIU Li-na~1,WANG Yu-xia~2,ClREN Zhuo-ga~2 (1.Horticulture of College,Sichuan Agricultural University,Ya'an,Sichuan 625014;2.Institute of Vegetables,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lasa,Tibet 850030;3.Maize Research Institute,Sichuan Agricultural University,Ya'an,Sichuan 625014);Optimization for SRAP-PCR System and Selection of Primers on Prunusmira Koehne from Tibet[J];Northern Horticulture;2011-02
4 WEI Bingqiang ( Vegetable Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070 );Progress of Selectable Marker-free Transgenic Plant[J];Journal of Changjiang Vegetables;2009-10
5 LI Li1,YANG Min-sheng1,LIANG Hai-yong1,WANG Jin-mao1,ZHANG Er-liang2(1.College of Forestry,Agricultural University of Hebei,Baoding 071000,China;2.Kelegou Forestry,State Forest Farm Authority of Mulan Centre Paddock,Chengde 068453,China);Analysis of the insect-resistance of the triploid Chinese white poplar transformed with two insect-resistant genes[J];Journal of Agricultural University of Hebei;2009-02
6 Zhang Xiuchun~1 Gou Liqiong~(1,2 *) Wu Kunxin~1 Lin Junyang~3 Lin Junfang~(1,2)(1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of TropicalAgricultural Sciences.Haikou 571101;2.Department of Bioengineering,College of FoodSciences,South China Agricultural University.Guangzhou 510640;3.Putian City'sInstitute of Agricultural Sciences.Putian,351100);AGROBACTERIUM-MEDIATED TRANSFORATION OF SOYBEAN WITH THE EXPRESSION VECTOR CARRYING TWO SEPARATE T-DNAs[J];Soybean Science;2005-04
7 Zhang Xiuchun~1 Peng Ming~1 Wu Kunxin~1 Guo Liqiong~(1,2) Lin Junyang Lin Junfang~(1,2) (1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101;2 Department of Bioengineering,College of Food Sciences,South China Agricultural University,Guangzhou 510640;3 Putian City's Institute of Agricultural Sciences,Putian 351100);GENERATING MARKER-FREE TRANSGENIC SOYBEAN PLANTS BY AGROBACTERIUM-MEDIATED TRANSFORMATION WITH DOUBLE T-DNA BINARY VECTOR[J];Soybean Science;2006-04
8 CHEN Rui-feng,ZHANG Jun-yi(Department of Bioengineering & Biotechnology,Huaqiao University,Xiamen,Fujian 361021,China);Genetic diversity of Liriope muscari(Decn.) Bailey detected by using ISSR markers[J];Fujian Journal of Agricultural Sciences;2010-03
9 Li Guimin Song Kai Liu Lixia Xu Shoumin*School of Life Sciences, Northeast Normal University, Changchun, 130024;Studies of Co-transformation for the Elimination of Selective Marker Genes from Transgenic Plants[J];Molecular Plant Breeding;2004-06
10 Qi Dengfeng Zou Liangping Li Ping* Wang Shiquan Li Shuangcheng Zhang Qijun Jiang HongRice Research Institute of Sichuan Agricultural University, Chengdu, 611130;Introduction of Lysine-rich Protein Gene sb401 into Rice by Agrobacterium tumefactions-mediated Method[J];Molecular Plant Breeding;2005-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Fang (Qinghai Academy of Agriculture and Forestry, Xining, Qinghai 810016);Study on DNA Extraction in Dry Barley Seed[J];Journal of Anhui Agricultural Sciences;2007-26
2 Zhang Xiuchun~1 Gou Liqiong~(1,2 *) Wu Kunxin~1 Lin Junyang~3 Lin Junfang~(1,2)(1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of TropicalAgricultural Sciences.Haikou 571101;2.Department of Bioengineering,College of FoodSciences,South China Agricultural University.Guangzhou 510640;3.Putian City'sInstitute of Agricultural Sciences.Putian,351100);AGROBACTERIUM-MEDIATED TRANSFORATION OF SOYBEAN WITH THE EXPRESSION VECTOR CARRYING TWO SEPARATE T-DNAs[J];Soybean Science;2005-04
3 Zhu Yu, Zhu Zhen, Xu Honglin (Institute of Genetics , Chinese Academy of Sciences , Beijing 100101)$$$$;Cloning and Sequencing of Snowdrop Lectin cDNA and Polymorphism Analysis[J];HIGH TECHNOLOGY LETTERS;1999-09
4 An Hanbing , Zhu Zhen, Li Huifen, Wu Qian, Zhou Zhaolan, Xu Honglin, Xu Junwang, An Lijia  , Li Xianghui (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101) ( * Department of Biology, Liaoning Normal University, Dalian 116;Obtaining Fertile Transgenic Plants of Resistant insect Rice (Oryza sativa L.) via Particle Bombardment[J];High Technology Letters;2001-02
5 LU Lu1,2, ZHAO Chang-zeng2, LU Ting3 (1Department of Horticulture, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070 China; 2Institute of Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, Hainan 571101 China; 3Department of Horticulture, Xinjiang Agricultural University, Urumqi, Xinjiang 830052 China);Construction of an antisense CM-ACO1 gene plant expression vector and its utilization in transformation of tobacco[J];Journal of Fruit Science;2005-05
6 YAO Qing-rong1, 2, ZHAO Chang-zeng1,WANG Wen-quan2 (1. Department of Horticulture, Gansu Agricultural University,Gansu, Lanzhou 730070;2. National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101, China);Extraction of genomic DNA and optimization of SSR amplified condition in litchi[J];Journal of Gansu Agricultural University;2004-02
7 JianYuyu1  WuXingrong1  MoHaokue1  Chen Fengzhen1 Dong Chun2  Huang Ziran3(1 Genetic Engineering Laboratory, South China Agric. Univ., Guangzhou, 510642; 2 College of Natural Resources & Environment; 3 Dept. of Sericulture,South;TRANSFER OF CECROPIN B GENE INTO RICE (Oryza sativa L.) BY THE BIOLISTIC METHOD AND TRANSGENIC PROGENY LINE FOR RESISTANCE TO BACTERIAL BLIGHT[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1997-04
8 KONG Xiu-qin, DAI Wei-min, GE Hai-tao, et al. (Department of Life Science, Nanjing University, Nanjing 210093, China);Studies on the hypolipidemic effects of gamma-linolenic acid methyl ester derived from Spirulina maxima[J];Chinese Journal of Marine Drugs;2003-06
9 ZHANG Ning,WANG Feng shan (Institute of Biochemic and Biotechnological Drugs,School of Pharmacy , Shandong University,Jinan 250012, China);Advances of DNA extraction methods[J];Chinese Journal of Marine Drugs;2004-02
10 Zhang Rong Wang Guoying Zhang Xiaohong Zhao Huji National Key Labs for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, Beijing 100094;Agrobacterium tumefaciens Mediated Maize Transformation[J];Journal of Agricultural Biotechnology;2001-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved