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《Soybean Science》 2013-06
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Study on Screening Concentration of Wide Adaptability Herbicide-Resistant bar-Transgenic Soybean

LI zhi;WANG Shu-ming;LIU Jia;MENG Fan-mei;YI Zhi-gang;DONG Zhi-min;Soybean Research Institute,Jilin Academy of Agricultural Sciences;National Engineering Research Center for Soybean;  
Glufosinate proves to be effective and convenient in screening transgenic soybean plants. To improve the screening efficiency of transgenic positive plants,the glufosinate screening concentrations were determined by two methods(daubing and spraying) in the greenhouse and field,with six common soybean transgenic receptor varieties as materials. The suitable glufosinate screening concentrations for daubing and spraying were 100-150 and 700-750 mg·L1,respectively,when determined by leaves color changes and mortality rate of soybean plants. Then the availability was tested by T 1 plants of soybean transgenic lines with bar gene,the accuracy rate was 61. 3% and 73. 9%,respectively. The results would facilitate the screening of glufosinate-resistant transgenic soybean progenies.
【Fund】: 转基因生物新品种培育重大专项(2013ZX08004-001-05;2014ZX08004-002B-003)
【CateGory Index】: S451.224
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