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《SOYBEAN SCIENCE》 1996-02
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PRILIMINARY STUDY ON IDENTIFICATION OF RAPD MARKER ASSOCIATED WITH RESISTANCE TO RACE 4 OF HETERODFRA GLYCINES

Yan Qingshang; Wang Lan; Li Ying; Wang Lianzheng; Chen Pinshan(Institute of Crop Breeding and Cultivation,CAAS, Beijing 100081)  
Applicating the randomly amplified polymorphic DNA(RAPD) techniquewe analysedeight soybean cultivars(lines), which included two resistant lines, 1259Y with yellow seed coatand 1259B with bicolor seed coat, and their resistant parent, Huipizhiheidou, susceptible parent,Jinyi 9, other two resistant varieties, Yuanboheidou and P1437654, other two susceptible cultivars, Ludou 1 and Ludou 7, to race 4 of Heterodera glycines for their amplified products.Among33 primers, one primer, OPG04, amplified a DNA fragment which specifically existed in theproduucts of all five resistant varieties and lines, and not found in these of three susceptible cultivars (including susceptible parent).
【Key Words】： Soybean Heterodera glycines RAPD technique
【CateGory Index】： S435.651
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