POLYMORPHISMS AND CLUSTER ANALYSIS OF CULTIVATED SOYBEANS WITH RAPD

Zhang Zhiyong Chen Shouyi (Institute of Genetics, Academia Sinica, 100101) Gai Junyi Hu Yunzhu Zhi Haijian (Soybean Research Institute, Nanjing Agricultural University,210095)  

In present study, twenty-one soybean accessions with host resistance to different SMV strains and 7 susceptible cultivars or lines were analysed for their genetic diversity using RAPD. Twenty 10-mer random primers were used to amplify the total DNA from 28 soybean accessions. The reults showed that repriducible amplified products could be got with 18 primers. The length of amplification products are between 0.6-4.6kb. Totally 165 RAPD bands were identified, of which, 104 bands were commom in all the accessions and 61 bands were polymorphic between any two accessions. The polymorphic bands accounted for 37% of the total amplified bands.These primers could be divided into six groups.1) no amplification products; 2) no polymorphisms between amplification products; 3) polymorphic bands accounted for less than 15% of the total bands; 4) polymorphisms between 15%-30%; 5) polymorphisms between 30%-50%; 6) polymophisms above 50%. Based on the genetic distance matrix, cluster analysis were conducted. The results showed that these soybean accessions could be divided into five groups. Cultivars and lines clustered together in any group or subgroup were consistent with their pedigree and geographical distribution.

【Key Words】： Cultivated soybeans RAPD Polymorphisms Cluster analysis
【CateGory Index】： Q75,S565.1

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