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《CHINESE JOURNAL OF CONTEMPORARY PEDIATRICS》 2000-04
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Delayed Cell Death Following Hypoxic-Ischemic Injury in Neonatal Rats

ZHOU Wei, WU Sheng Mei, CHEN Hui Jin, et al. Department of Pediatrics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China  
Objective To determine whether or not apoptosis occurs in the delayed cell death following hypoxic ischemic injury, and assess the sensibility and specificity of various techniques. Methods Using HE staining and electromicroscopic technique, in situ end labeling (ISEL) and DNA electrophoresis, we observed the histological features of apoptotic cells in the cerebral cortex and hippocampus, and compared the ipsilateral hemisphere at various time points following hypoxic ischemia (HI) with that of the sham group. Results In the cerebral cortex and hippocampus at the ipsilateral hemisphere in neonatal rat models of HI, light and electronic microscopy exhibited cell shrinkage, chromatin condensation, and apoptotic bodies. ISEL and DNA electrophoresis also provided evidence of DNA fragmentation. It was found that apoptotic cell death generally began 6~12 h after the initiating insult, and reached the peak at 24 h in the cortex and hippocampus. Conclusions Cerebral HI can induce neuronal apoptosis in neonatal rats. Apoptosis is mainly involved in the delayed cell death following hypoxic ischemic injury except for the existence of necrosis. Apoptosis appears to be a major form of delayed cell death during the selective neuronal loss following hypoxic ischemic injury. Owing to the limitation of different techniques, it is necessary to adopt several ways to determine the existence of apoptosis.
【CateGory Index】: R722.1
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【References】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Wei, LAI Jian Pu, LV Hui, et al. Department of Neonatology, Guangzhou Children's Hospital, Guangzhou 510120, China;Effect of Early Medication Intervention on Neonatal Brain Damage Following Severe Perinatal Asphyxia[J];Chinese Journal of Contemporary Pediatrics;2002-06
2 ZHANG Xiao-lei, XU Jin-chen, LIU Chen-zhou, et al. Department of Pediatrics, The Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001, China;The Effect of N-acetylcysteine on ICAM-1 Expression and Apoptosis in Neonatal Rats Brain after Hypoxic and Ischemic Brain Damage[J];Journal of Chinese Physician;2005-06
3 PEI Huai-teng (Hospital of Xi’an Electric Group, Shanxi Xi’an 710077, China);The optimal time for prevention of neonatal hypoxic-ischemic encephalopathy with comprehensive treatment[J];Journal of Pediatric Pharmacy;2007-02
4 ZHANG Xiao-lei, XU Jin-cheng , TAN Jian-xin , WANG Xiao-peng. (Department of Pediatrics Affiliated Hospital, Guangdong Medical College ,Zhanjiang, Guangdong 524001, China);Changes of intercellular adhesion moleculel-1 and apoptosis and its correlation in neonatal rats with hypoxic ischemic brain damage[J];Chinese Journal of Child Health Care;2005-02
5 LIN Jiu mao,CHEN Xiao feng,ZHENG Liang pu\;(Central Laboratory,FCTCM,Fuzhou,Fujian 350003,China);;Experimental Study on Cerbral Apoptosis in Senile Rats[J];Journal of Fujian College of Traditional Chinese Medicine;2002-03
6 GUO Wei-zhong1 LI Dan1 LUO Hai-biao2 1Pediatrics department in nanhan maternity and children hospital, guangzhou university of traditional Chinese Medicine Foshan, Foshan 528200 Guandong China 2Pediatrics department in the first Hospital in Shaoguan,Shaoguan 512000 Guandong China;Clinical effect of naloxone in the treatment of neonatal severe asphyxia[J];International Medicine & Health Guidance News;2008-17
7 ZHUO Ming,CHEN Hong-lian,LIANG Chun-Jie,HUAN Da-Ming,YE Guo-jie,LIN Zhan,CAI Na-li (1 Department of Emergency,2 Department of Pediatrics,Zhanjiang Central People's Hospital,Zhanjiang 524037,China;3 Department of Experiment,4 Department of Pediatrics,the Affiliated Hospital of Guangdong Medical College,Zhanjiang 524000,China);The influences of prognosis of early therapy of astragalus injection in neonates with hypoxic ischemic encephalopathy[J];Guangxi Medical Journal;2009-04
8 Li Zhuanlei(Ninling county health institute for women and children,476700,Henan,China);Early intervention in newborns with hypoxie-ischemic encephalopathy[J];Journal of Clinical Psychosomatic Diseases;2005-03
9 TANG Yao-bin,SHAN Wei-hua,FENG Fei-yue, et al.Departent of Pediatrics,Nanhai Hospital,Guangdong 528200,China;Protective role of Naloxone in brain cell of newborn with severe birth asphyxia[J];Pediatric Emergency Medicine;2004-06
10 CHEN Zhen-xiang,ZHU Hong-wei,SHEN Huai-yun,et al.Department of Pediatrics,the First Affiliated Hospital of Bengbu Medical College.Anhui 233004,China;Influence of 1,6-FDP on Curative Effect and Blood Gas in Patients with Neonatal Hypoxic-Ischemic Encephalopathy[J];Applied Journal of General Practice;2008-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GUO Gai-hui,FU Ren-jie (China-Japan Friendship Hospital,Beijing 100029,China);SYNDROME CHARACTERISTICS OF 170 MIDDLE AGED AND SENILE PATIENTS WITH ACUTE CEREBRAL INFARCTION[J];Journal of Anhui Traditional Chinese Medical College;2002-06
2 LU Jian-ming, MA Yun-bao, GAO Lei-hua (Changshu No.2 Hospital Affiliated to Medical College of Yangzhou University, Changshu 215500, China);Significance of Determination of ET, CGRP, MDA and SOD in Patients with Ischemic Cerebral Apoplexy[J];Labeled Immunoassays and Clinical Medicine;2005-01
3 Wang Ronghui 1) Liu Guihua 1) Hu Qi 2) Zhang Yimin 1);Effects of Hypoxic Training on LDH and MDH Activities of Skeletal Muscles in Rats[J];JOURNAL OF BEIJING UNIVERSITY OF PHYSICAL EDVCATION;1998-03
4 LIU Ming~1, ZHANG Guo-qing~1, MA Xian-ying~2 (1.Shandong Sports Training and Technology College, Jinan 250014, Shandong China; 2. Shandong Financial College, Jinan 250002, Shandong, China);Research on Oxygen-transport Ability of Swimmers as the Effect of Simulating Altitude Training[J];Journal of Beijing University of Physical Education;2004-06
5 YIN Wei-na, ZHANG Chuan-xin Department of P. E. Qufu Normal University,Qufu 273165; Sports-Science Department of Taishan University ,Tai'an 271000,China;The Development and Studying of Plateau Training[J];Journal of Beijing Teachers College of Physical Education;2005-03
6 LI Xing gui,JIA Yan jie,YANG Yu jia,et al. (1.Department of Neurology,the Fifth People′s Hospital of Chongqing,Chongqing 400062,China;2.Neurology Institute. the First Affiliated Hospital,Chongqing University of Medical Sciences Chongqing 400016,China; 3.Deparmtnet of Pediatrics,Xiangya Hospital,Zhongnan University,Changsha 410008,China);Gene therapy for hypoxic-ischemic brain damage by neurotrophin-4-engineered bone marrow stromal cells transplantation[J];Chongqing Medical Journal;2004-03
7 JIA Yan-Jie, YANG Yu-Jia,SONG Jian-Hui, et al.(Department of Pediatrics, Xiangya Hospital,Central South University, Changsha 410008, China);Isolation and Cultivation of Neural Stem Cells in neonatal Rats[J];Chinese Journal of Contemporary Pediatrics;2002-02
8 Xiao-He YU, Yu-Jia YANG, Xia WANG, Le ZHONGDepartment of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China;Hyperbaric oxygen therapy protects rats from hypoxic-ischemic brain damage[J];The Chinese Journal of Contemporary Pediatrics;2004-06
9 Le ZHONG, Xia WANG, Xiao-He YU, Yu-Jia YANG. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China;Long-lasting behavioral alterations in neonatal rats after hypoxic-ischemic brain damage[J];The Chinese Journal of Contemporary Pediatrics;2005-03
10 WANG Lai-Shuan, YU Li-Jun, SHAO Xiao-Mei. Department of Pediatrics, Children's Hospital of Fudan University, Shanghai 200032, China;Mild hypothermia attenuates neuronal apoptosis after cerebral hypoxia-ischemia in neonatal rats[J];Chinese Journal of Contemporary Pediatrics;2007-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YI Jun,JIA Qu,HU Qing,LIAO Zheng-hui,ZHANG Jian(Dali Teaching Hospital of Dali University,Dali,Yunnan 671000,China);Assessment of Prognosis of Hypoxic-Ischemic Encephalopathy (HIE) after Early Intervention[J];Journal of Dali University;2008-10
2 PEI Huai-teng (Hospital of Xi’an Electric Group, Shanxi Xi’an 710077, China);The optimal time for prevention of neonatal hypoxic-ischemic encephalopathy with comprehensive treatment[J];Journal of Pediatric Pharmacy;2007-02
3 GUO Wei-zhong1 LI Dan1 LUO Hai-biao2 1Pediatrics department in nanhan maternity and children hospital, guangzhou university of traditional Chinese Medicine Foshan, Foshan 528200 Guandong China 2Pediatrics department in the first Hospital in Shaoguan,Shaoguan 512000 Guandong China;Clinical effect of naloxone in the treatment of neonatal severe asphyxia[J];International Medicine & Health Guidance News;2008-17
4 LI Ya,GAO Yu-li. Pediatric Yueyang City Maternity and Child Care Hospitals Yueyang,Hunan 414000,China;Analysis of integrated intervention and prognosis for infants' hypoxic-ischemic encephalopathy(HIE)[J];Journal of Public Health and Preventive Medicine;2009-05
5 TANG Li(Pharmaceutical Department,the First Affiliated Hospital,Nanhua University,Hengyang,Hunan 421001,China);Clinical Observation on Curative Effect of Naloxone for Neonate Hypoxic-ischemic Encephalopathy(HIE)[J];Journal of Nanhua University(Medical Edition);2007-06
6 LI Qiao-hong, ZOU Yong-Rong, GONG Fang, et al. Department of Clinical Labortory, the People's Hospital of Yaan City, Yaan 625000, China;Early intervention and the prognosis evaluation of neonates with hypoxic-ischemic encephalopathy[J];Laboratory Medicine and Clinic;2007-09
7 DING Xiaoting CHANG Qing YANG Xiulan KANG Mingli(General Hospital with the Coal Group,SHAN Xi,DA Tong,037003);Naloxone treatment of neonatal hypoxic-ischemic encephalopathy Analysis[J];Chinese Journal of Ethnomedicine and Ethnopharmacy;2009-10
8 CHEN Zhen-xiang,ZHU Hong-wei,SHEN Huai-yun,et al.Department of Pediatrics,the First Affiliated Hospital of Bengbu Medical College.Anhui 233004,China;Influence of 1,6-FDP on Curative Effect and Blood Gas in Patients with Neonatal Hypoxic-Ischemic Encephalopathy[J];Applied Journal of General Practice;2008-01
9 XIE Sihua, ZHOU Ling, LAO Bing, et al;BRAINPOWER INVESTIGATION OF NEWBORNS WITH MODERATE OR SEVERE ANOXIA ISCHEMIC ENCEPHALOPATHY[J];Modern Hospital;2007-08
10 ;Observation on Effect of Early Use of Laloxone on Neonatal Hypoxic-Ischemic Encephalopathy[J];Modern Diagnosis & Treatment;2004-03
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