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《The Chinese Journal of Contemporary Pediatrics》 2004-06
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The effect of matrine on Bcl-2 expression in acute lymphoblastic leukemia cells

Zun-Ke QIN, Xiao-Cheng YIN. The People's Hospital of Zhangjiajie, Zhangjiajie, Hunan 427000,China  
Objective Matraine, a main ingredient of sophora, can inhibit the proliferation of many tumor cells such as K562 and HL-60. This study was designed to investigate the effect of matrine on acute lymphoblastic leukemia (ALL) cells and its possible mechanisms. Methods The samples were obtained from 24 children with initial ALL. The ALL cells were co-cultured with matrine. The inhibition effect of the proliferation of ALL cells was detected by the MTT assay. A flow cytometer (FCM) was used to detect the expression of Bcl-2 in ALL cells. Results Compared with the controls without matrine treatment ( 0.84± 0.27), the OD values (reflecting the survival cell amount) were reduced significantly after 48 hrs of different concentrations of matrine incubation ( 0.73± 0.16 at 0.1 mg/ml, 0.58± 0.15 at 0.2 mg/ml, 0.32± 0.16 at 0.5 mg/ml, and 0.24± 0.14 at 1.0 mg/ml). The positive rate of Bcl-2 protein was down-regulated significantly from ( 25.0± 5.8)% in the untreated controls to ( 21.3± 6.1)% in the cells treated with 0.1 mg/ml matrine, and ( 18.2± 3.7)% for 0.2 mg/ml, ( 14.5± 4.0)% for 0.5 mg/ml and ( 10.0± 3.2)% for 1.0 mg/ml at 48 hrs of incubation. Conclusions Matraine can inhibit the proliferation of ALL cells, and the possible mechanism may be associated with the down-regulated Bcl-2 expression in ALL cells.
【Fund】: 湖南省卫生厅项目基金课题 (编号 2 0 0 1-Y -90 )
【CateGory Index】: R733.7
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【References】
Chinese Journal Full-text Database 4 Hits
1 DAI Bi-tao,XU You-hua(Children’s Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China);Advances in study of effect of Matrin onleukemia cell[J];Journal of Pediatric Pharmacy;2006-04
2 Zhu Shi-yan1,Gan Ping2,Cao Yuan-guo1,Cheng Zhuo1(1.Department of Nephrology,Longjiang Hospital,Foshan Guangdong 528318,China;2.Department of Nephrology,Affiliated Hospital of Guiyang Medical College,Guiyang Guizhou 550001,China);Effect of matrine on high glucose-induced NF-κB p65 overexpression in rat peritoneal mesothelial cells[J];Clinical Journal of Medical Officers;2010-05
3 SU Yong-zhong,XIE Jian-ying,ZHU Wen-jie,et al.Department of Hematology,The First Affiliated Hospital of Shantou University Medical College,Shantou Guangdong 515041,China.;The therapeutic effect of matrine combined with chemotherapy in treatment of acute lymphocytic leukemia[J];Journal of Clinical and Experimental Medicine;2008-07
4 WANG Zhi-hong,CAO Yong-bin,WU Xiao-xiong,MA Jian,XU Li-xin,LIU Bei,LIU Zhou-yang,YAN Bei (The First Affiliated Hospital,General Hospital of PLA,Beijing 100037,China);Evaluation of Compound Radix Sophorae Flavescentis Injection in Adjuvant Chemotherapy for Malignant Hematopathy[J];Evaluation and Analysis of Drug-Use in Hospitals of China;2008-11
【Citations】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Yan, JIANG Ji kai*, LIU Xiao shan, et al Department of Clinical Biochemistry, Chongqing University of Medical Sciences, Chongqing 400016, P R China;Differentiation and apoptosis in K562 erythroleukemia cells induced by matrine[J];CHINESE JOURNAL OF CANCER;2000-08
2 TAN Meng qun 1, LUO Zhi yong 2, WANG Qi ru 1, JIANG De zhao 1 1Laboratory of Blood Physiology, Hunan Medical University(Changsha 410078); 2Molecular Biology Research Center, Hunan Medical University(Changsha 410078) [;The anti-leukemia effect of Sophora flavescens and its mechanism[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;2000-05
3 ZHANG Bai hong,YUE Hong yun,LI Xin ming et al. Department of Oncology,Lanzhou Military General Hospital,Lanzhou 730050〔;Effect of matrine on expression of Bcl-2 in gastric carcinoma cells[J];CHINESE JOURNAL OF CLINKAL ONCOLOGY AND REHABILITATION;2000-03
4 Xu Jianguo, Ma Junying, Yang Guisheng, Ren Liansheng, Guo Yadong Zhang Hongxiang(Shanxi Institute of Cancer Research, Taiyuan 030013);Differentiation of Human Leukemia Cell (HL-60) Induced by Sophora flavescens Ait. Decoction[J];China Journal of Chinese Materia Medica;1990-10
5 Zhang Liping, Jiang Jikai, Tan Rongan, et al. Department of Clinical Biochemistry, Chongqing University of Medical Science, Chongqing 400046;Effects of matrine on telomerase activity and cell cycle in K562 cell[J];CHINESE JOURNAL OF ONCOLOGY;1998-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SONG Qi-Fang1,2,QU Yan-Chun2,ZHENG Hong-Bo1,ZHANG Gao-Hua2,LIN Hong-Gang2,YANG Jin-Liang2 1. School of Life Sciences,Sichuan University,Chengdu,Sichuan,610064,P. R. China 2. State Key Laboratory of Biotherapy,School of Life Science,West China Hospital,Sichuan University,Chengdu,Sichuan,610041,P. R. China;Differentiation of Erythroleukemia K562 Cells Induced by Piperine[J];Chinese Journal of Cancer;2008-06
2 Chen Tingmei, Zhu Bide, Luo Yunping, et al Chong qing medical university(Chong qing,400016);EFFECT OF TOTAL SAPONINS OF THREE CHINESE TRADITIONAL MEDICINES ON INDUCED DIFFERENTIATION OF K 562 CELLS[J];JOURNAL OF LEUKEMIA;1999-01
3 Luo Wenji(The First Hospital of People of Xiaoshan County; Zhejiang; The Hospital of TCM of Zhejiang Province, Hang Zhou);Experimental Research of Sophocarpidine Inducing Leukemia Cell HL-60 into Differentiation[J];China Journal of Traditional Chinese Medicine and Pharmacy;2001-05
4 CHENG Guang-you,TANG Xiao-jie(Forestry College,Beihua University,Jilin,Jilin 132013,China);Study on Tissue Culture and Rapid Propagation of Sophora flavescens Ait.[J];Acta Botanica Boreali-Occidentalia Sinica;2007-05
5 ZHANG Yan Jun 1,XIA Tian 2,ZHAO Jian Bin 2 1Oncology center, 2Department of Traditional chinese Medicine,Xijing Hospital, Fourth Military Medical University,Xi'an 710033;Effects of Matrine on the differentiation of SMMC 7721 cell lines[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-03
6 DAI Bi-tao,XU You-hua(Children’s Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China);Advances in study of effect of Matrin onleukemia cell[J];Journal of Pediatric Pharmacy;2006-04
7 SHEN Xiao-dong, SONG Guan-bin, YAN Run-bin, YANG Yang (Key Laboratory for Biomechanics and Tissue Engineering Under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China);Research Progress of Matrine and Oxymatirne in the Anti-tumor Mechanism[J];Journal of Chongqing University(Natural Science Edition);2005-06
8 XIANG Qiu,WANG Jianhong,HUANG Yanhong,et al.Biotechnology Research Institute,Guilin Medical College,Guilin 541004,Chian;Effect of Sophora Slavescens on Acute Myloid Leukemia[J];Acta Medicinae Sinica;2004-01
9 XIE Qiang,ZHAO Dang,LIU Xing-jing,HUANG Zuo-ping,ZOU Bing-xin,Wang Li-gen.Department of Oncology,Guangdong Provincial Corp Hospital,Chinese People's Armed Police Forces,Guangzhou 510507,China;;Efficacy of compound matrine injection plus intensity-modulated radiation treating for nasopharyngeal carcinoma patients[J];International Journal of Internal Medicine;2008-03
10 Sun Jian,Yao Ping,He Xiaohua(Department of Digestion of Xuzhou People's Hospital,Xuzhou 221002,China);The Clinical Observation of the Effects of Compound Radix Sophorae Flavescentis Injection Combined with Radio Frequency Therapy on Advanced Esophageal Cancer[J];Journal of Basic and Clinical Oncology;2006-05
China Proceedings of conference Full-text Database 1 Hits
1 ;Result observation on treating Ⅲ stage of stomach cancer with Fu-fang Ku-shen inject plus low dosage of chemotherapy[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HE Feng tian, ZHU Xi hua, HUANG Yun hui Department of Immunology, The Third Military Medical University, Chongqing 400038, P R China;Influence of antisense bcl-2 gene on survival of mononuclear leukemia cell U937 and the sensitivity of the cell to chemotherapeutic drugs[J];CHINESE JOURNAL OF CANCER;2000-04
2 HE Dong-mei ; ZHANG Huan (Institute of Hematology, Jinan University Medical College , Guangzhou 510632, P R China);Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in K562 cells[J];CHINESE JOURNAL OF CANCER;2000-07
3 ZHANG Yan, JIANG Ji kai*, LIU Xiao shan, et al Department of Clinical Biochemistry, Chongqing University of Medical Sciences, Chongqing 400016, P R China;Differentiation and apoptosis in K562 erythroleukemia cells induced by matrine[J];CHINESE JOURNAL OF CANCER;2000-08
4 LI Xu- fen1* , ZHANG Su- zhan1, ZHENG Shu2, ZHANG Xing2 1.Department of Oncology,the Second Affiliated Hospital School of Medicine, Zhejiang University,Hangzhou 310009,P.R.China 2.Cancer Institute,Zhejiang University, Hangzhou 310009, P.R.China;Effects of Matrine on hTERT- mRNA Expression and Telomerase Activity in K562 Cells[J];Chinese Journal of Cancer;2001-04
5 SI Wei ke*, GAO Li hong, LIU Bing, CHENG An, LI Peng, YAO JieDepartment of Clinical Biochemistry, the Third Military Medical University, Chongqing 400038, P.R.China;Regulation of G_1 Phase Cell Cycle Regulation Factors in Differentiation and Apoptosis of Matrine Induced Hepatoma Cells[J];Chinese Journal of Cancer;2001-08
6 HE Yu juan*,JIANG Ji kai,OU Yi heng,LIU Bei zhong,LIU Xiao shan,ZHANG Yan,MA Lin di,TU Zhi guangDepartment of Clinical Bio chemistry,Chongqing University of Medical Sciences,Chongqing 400016,P.R.China;Matrine Affects Early Expression ofProto-oncogenes in K562 Cells[J];Chinese Journal of Cancer;2002-04
7 HE Dong mei, ZHANG Huan*Institute of Hematology,Jinan University Medical College,Guangzhou 510632,P.R.China;Telomerase Gene Antisense Oligodeoxynucleotide Down-regulates Telomerase Activity in HL-60 Cells[J];Chinese Journal of Cancer;2002-10
8 LIU Bei zhong1, JIANG Ji kai1, HE Yu juan1, ZHANG Yan1, LIU Xiao shan21. Department of Clinical Biochemistry,Chongqing Medical University,Chongqing 400016,P.R.China;2. Department of Hematology,College of Clinical Medicine,Chongqing Medical University,Chongqing 400016,P.R.China;Effects of Matrine on the Activity of Protein Tyrosine Kinase and Phosphatase in K562 Cells[J];Chinese Journal of Cancer;2002-12
9 SHANG Zhenchuan, SONG Tingting, SUN Bingzhong, et al The No.401 Hospital of PLA, Qingdao 266071; [;EFFECT OF ANTISENSE OLIGODEOXYNUCLEOTIDE ON SUSCEPTIBILITY OF ACUTE LEUKEMIA CELLS TO CHEMOTHERAPEUTIC AGENTS[J];ACTA ACADIMAE MEDICINAE QINGDAO;2000-01
10 Huang Xueqiang, Ling Changquan, Song Tianbin, et al Changhai Hospital, Second Military Medical University, Shanghai(200433);Study on Apoptosis of Human Acute T Lymphocyte Leukemia Cell Line 6t-CEM lnduced by Norcantharidin[J];Journal of Zhejiang College of Tcm;2001-05
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 Zheng Xiaolin1, 2, Liu Hongying1, Tang Wei3, Pi Xitian1, 2, Hou Wensheng1, Zhou Chengwen1 (1College of Bioengineering, 2Microsystem Research Center, Chongqing University, Chongqing, 400030; 3Department of Equipment, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China);Pharmacokinetics of matrine in proximal small intestine based on remote controlled capsule[J];Acta Academiae Medicinae Militaris Tertiae;2010-06
2 ZHU Xiao-wei1,BAO Jin-rong2,BU Ren1 (1.Pharmaceutical College,Inner Mongolia Medical College,Hohhot 010110,China;2.College of Chemistry & Chemical Engineering,Inner Mongolia University,Hohhot 010021,China);Progress on the anti-tumor activities of matrine and oxymatrine[J];Chemical Reagents;2010-01
3 LI-Chun-mei,ZHANG Zong-jian,SHI Yan(The Third Affilated Hospital of Inner Monglia Medical College,Baotou 014010,China);The Injection on Clinical Application of Compound Sophora[J];Journal of Diseases Monitor & Control;2010-06
4 LU Qian-wei~1,CHEN Jian-bin~(2*),TANG Wei-xue~3,LIAO Yang~2, GE Qun-fang~2,YANG Ze-song~2(1.Department of Oncology and Hematology,No.9 Chongqing People's Hospital,Chongqing 400700;2.Department of Hematology,No.1 Hospital(Affiliated) to Chongqing Medical University,Chongqing 400016;3.Department of Pathophysiology,Chongqing Medical University,Chongqing 400016,China);Influence of matrine on cyclin in U937 cell strain[J];Chinese Traditional Patent Medicine;2009-11
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Yan Jun 1,XIA Tian 2,ZHAO Jian Bin 2 1Oncology center, 2Department of Traditional chinese Medicine,Xijing Hospital, Fourth Military Medical University,Xi'an 710033;Effects of Matrine on the differentiation of SMMC 7721 cell lines[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-03
2 Xu Jianguo, Ma Junying, Yang Guisheng, Ren Liansheng, Guo Yadong Zhang Hongxiang(Shanxi Institute of Cancer Research, Taiyuan 030013);Differentiation of Human Leukemia Cell (HL-60) Induced by Sophora flavescens Ait. Decoction[J];China Journal of Chinese Materia Medica;1990-10
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