Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Contemporary Pediatrics》 2006-02
Add to Favorite Get Latest Update

Transdifferentiation and collagen-synthesis effects of exogenous connective tissue growth factor on renal tubular epithelial cells in vitro

QIAN Ming,GAN Wei-Hua,CHEN Rong-Hua,PAN Xiao-Qin,FEI Li.Institute of Pediatrics,Nanjing Medical University,Nanjing 210029,China  
Objective To investigate the effects of recombinal human connective tissue growth factor(rhCTGF) stimulation on epithelial-myofibroblast transdifferentiation(EMT) and collagen-synthesis in human renal tubular epithelial cell line(HK2) in vitro.Methods The cultured HK2 cells were stimulated with rhCTGF of 5 ng/mL.The morphological changes were observed under an inverted microscope.The cells were collected at 0,3,6,12,24 and 48 hrs after rhCTGF stimulation.The expression of E-cadherin,α-smooth muscle actin(α-SMA),collagen Iα_1(Col Iα_1) and collagen IVα_1(Col IVα_1) mRNAs were detected by RT-PCR.Results rhCTGF stimulation changed the HK2 cell appearance from oval to fusiform,down-regulated the E-cadherin mRNA expression and up-regulated α-SMA mRNA expression,but had no effects the Col Iα_1 and Col IVα_1 mRNA expression. Conclusions Exogenous CTGF can mediate the EMT but has no collagen-synthesis effects on HK2 cells.
【CateGory Index】: R692.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Ai-Qing, GAN Wei-Hua, DING Gui-Xia, GONG Jing. Department of Pediatric Nephrology, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China;Effects of exogenous connective tissue growth factor on collagen Ⅲ synthesis of human renal tubular epithelial cells[J];Chinese Journal of Contemporary Pediatrics;2008-02
2 CHENG Xue-qin~* BAO Hua-ying CHEN Ying PAN Xiao-qin FEI Li CHEN Rong-hua ~*Nanjing Children Hospital Affiliated to Nanjing Medical University,Nanjing 210008,China;Effects of core proteoglycan on the transdifferentiation of human renal tubular epithelial cell induced by transforming growth factor-β_1 in vitro[J];Chinese Journal of Pediatrics;2007-07
【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Chun, ZHU Zhong-hua, DENG An-guo. Division of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Effect of connective tissue growth factor on transdifferentiation of human renal tubular epithelial cell[J];Chinese Journal of Nephrology;2003-06
2 ZHANG Hai yan, LI You ji,DU Yong,LIANG Ming,LI Xiao yan,YU Xue qing,YE Ren gao . Department of Nephrology,The First Affiliated Hospital, Sun Yat sen University, Guangzhou 510080, China;Inhibition of transforming growth factor-β-induced collagen secretion by connective tissue growth factor antisense oligonucleotide in tubular epithelial cells[J];Chinese Journal of Nephrology;2004-02
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 ZHANG Ai-Qing1, GAN Wei-Hua1, PAN Xiao-Qin2, FEI Li2, GUO Mei2, CHEN Rong-Hua21Department of Pediatrics, Second Affiliated Hospital, Nanjing Medical University, Nanjing 210011, China, 2Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China;Effects of exogenous connective tissue growth factor on transdifferentiation and extracellular matrix synthesis of human renal tubular epithelial cells[J];Journal of the Fourth Military Medical University;2007-12
2 LANG Ming-Jian1,2, ZENG Qiu-Tang1, GUO Min1, YANG Han-Dong2, MIN Xin-Wen21Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China, 2Department of Cardiology, Dongfeng General Hospital, Yunyang Medical College, Shiyan 442008, China;Construction of recombinant shRNA-expressing vectors targeting to rat connective tissue growth factor and analysis of its effects[J];Journal of the Fourth Military Medical University;2008-01
3 SHI Hong,ZHOU Dun(Division of Nephrology,the First Affiliated Hospital,Jinzhou Medical College,Jinzhou 121000 China);Rosiglitazone Inhibits Renal Interstitial Fibrosis in a Rat Unilateral Ureteral Obstructive Model[J];Journal of Jinzhou Medical College;2006-05
4 XIAO Houqin,ZHANG Jiane,DING Guohua,et al.Department of Nephropathy,the Affiliated Taihe Hospital of Yunyang Medical College,Shiyan 442000,China;Urinary excretion rate of connective tissue growth factor in diabetic patients and its clinical significance[J];Journal of Clinical Internal Medicine;2004-08
5 LANG Mingjian1 ZENG Qiutang1 GUO Min1 YANG Handong2 MIN Xinwen2 (1Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China;2Department of Cardiology, Dongfeng General Hospital,Yunyang Medical College);Construction and identification of recombinant shRNA-expressing plasmid target to rat connective tissue growth factor[J];Journal of Clinical Cardiology;2007-01
6 ZHANG Yong-mei,WU Sheng-hua,LU Chao,REN Wei,TAO Hui-xian (Department of Pediatrics,the First Affiliated Hospital of NJMU,Nanjing 210029,China);Lipoxin A_4 inhibits epithelial-myofibroblast transdifferentiation of human renal tubular epithelial cells induced by CTGF[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2009-07
7 QIAN Ming,GAN Wei-hua,PAN Xiao-qin,FEI Li,GUO Mei,HUANG Yan-jun,XU Song-zhou,CHEN Rong-hua;Investigating the effect of exogenous and endogenous connective tissue growth factor on collagen-synthesis in renal tubular cells in vitro[J];Chinese Journal of Pathophysiology;2007-10
8 ZHANG Chun,ZH U Zhong-hua,DENG An-guo.Department of Nephrology,Union Hospital,Tongji Medica l College,Huazhong University of Science and Technology,Wuhan 430022,China;Effect of connective tissue growth factor antisense oligodeoxynucleotide on pl asminogen activator inhibitor-1 expression in renal tubular cells[J];Chinese Journal of Nephrology;2004-04
9 Zou Minshu Yu Jian Liu Xuemei Nie Guoming Liu Jing Department of pediatrics,Wuhan General Hospital of PLA,Wuhan 430070,China;Influence of Losartan on Transforming Growth Factor-β_1 and Connective Tissue Growth Factor in Rats with Focal Segmental Glomerulosclerosis[J];China Pharmacist;2008-01
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Mei, LI Xiao-Mei (Renal Division, Department of Medicine, First Hospital and Institute of Nephrology, Peking University, Beijing 100034);Signal Transduction on Transdifferentiation of Renal Tubular Epithelial Cell[J];Progress In Physiological Sciences;2004-03
2 GUO Hua, ZOU Wan-zhong. Department of Pathology, Health Science Center, Peking University, Beijing 100083, China;Transdifferentiation of tubular epithelial cells in tubulointerstitial fibrosis[J];Chinese Journal of Pathology;2003-04
3 WANG Hui-jun, ZHANG Zhi-gang, LIU Xue-guang, ZHANG Xiu-rong, CHEN Qi, GUO Mu-yi. Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China Corresponding author: GUO Mu-yi;The antagonistic effect on anti-thy-1 serum-induced nephritis of rats injected by decorin-transfected mesangial cells vector[J];Chinese Journal of Pathology;2003-05
4 WANG Wei-ming,CHEN Nan,LU Jian,CHEN Shi-shu,DONG De-chang. Department of Nephrology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China;Effect of decorin on the behavior of renal interstitial fibroblasts[J];Chinese Journal of Nephrology;2003-05
5 LI Jian, LI Xiaomei, WANG Haiyan. Institute of Nephrology, Renal Division, Department of Medicine, The First Teaching Hospital, Beijing Medical University, Beijing 100034, China;Human proximal tubular epithelial cells activated by low density lipoprotein promote phenotypic changes in renal interstitial fibroblasts[J];National Medical Journal of China;2000-10
6 ZHANG Chun, ZHU Zhong-hua, LIU Jian-she, YANG Xiao, DENG An-guo. Department of Nephrology, Union Hospital , Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Role of connective tissue growth factor in human renal tubular epithelial cell transdifferentiation in vitro[J];National Medical Journal of China;2005-41
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Cheng Qingli (Cheng QL),Chen Xiangmei (Chen XM),Li Feng (Li F), et al (Department of Nephrology,General Hospital of Chinese PLA,Beijing 100853) 39870321(96Q104);Distribution of ICAM1 antisense oligodeoxynucleotide and its inhibition effect on ICAM1 expression in mouse kidneys[J];CHINESE PHARMACEUTICAL JOURNAL;1999-08
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved