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《Journal of Chinese Physician》 2004-12
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The Apoptosis Induction Effect of Recombinant Caspase-3 on the Human Osteosarcoma Cell Line SOSP-9901

YU Bing, FAN Qing-yu, YAN Lu,et al. Orthopedics Oncology Institute of PLA, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China  
Objective To investigate the apoptosis induction effect of recombinant caspase-3 expression on osteosarcoma cell line SOSP-9901. Methods Recombinant caspase-3 gene was subcloned into the GFP reporter vector pEGFP-C1 to generate the expression vector pEGFP-caspase-3 by DNA recombinant technique. pEGFP-caspase-3 was transfected into human osteosarcoma cell line SOSP-9901 by Lipofectamine 2000. The expression of recombinant caspase-3 was determined by RT-PCR. The morphological changes of transfected cells were observed under flurescent and electronic microscope. The cell survival rate of transfected cells was assayed by MTT method. Results Recombinant caspase-3 gene could be stably expressed in the transfected SSOP-9901 cells. Recombinant caspase-3 could obviously induce SOSP-9901 apoptosis, and inhibit SSOP-9901 cell proliferation in vitro. Conclusion Recombinant caspase-3 could inhibit the growth of the osteosarcoma cell line SOSP-9901 and induce it into apoptosis.
【Fund】: 国家自然科学基金重点资助项目 (30 330 61 0 )
【CateGory Index】: R738.1
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【References】
Chinese Journal Full-text Database 2 Hits
1 GAO You-shui1,ZHANG Li-zhi1,QIAN Guang2,TONG Tian-lang1,HU Mu3,MEI Jiong1,CAI Xuan-song1(1.Department of Orthopedics,Tongji Hospital,Tongji University,Shanghai 200065;2.Shanghai the 5th People's Hospital,Shanghai 200240;3.Shanghai Pudong New Area People's Hospital,Shanghai 201200,China);Upregulation of caspase-3 expression and apoptosis of MG63 cell line induced by pSilencer-VEGF-siRNA transfection[J];Basic & Clinical Medicine;2009-04
2 LONG Hua,YUAN Hua,MA Baoan,et al.Department of Orthopedics,Tangdu Hospital,Fourth Military Medical University,Xi'an Shaanxi,710038,P.R.China;CONSTRUCTION OF RECOMBINANT ADENOVIRUS BEARING HUMAN TRANSFORMING GROWTH FACTOR β_1 AND BONE MORPHOGENETIC PROTEIN 7 GENES AND ITS DIRECTIONAL INDUCEMENT EFFECT ON MARROW STROMAL STEM CELLS[J];Chinese Journal of Reparative and Reconstructive Surgery;2006-12
【Citations】
Chinese Journal Full-text Database 1 Hits
1 JIA Lin Tao, YU Cui Juan, XU Yan Ming, ZHU Feng, PENG Wei Dan, SU Cheng Zhi, WANG Cheng Ji, YANG An Gang Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China;Construction and effect of caspase-3 fusion proteins and their apoptosis induction in HeLa cells[J];Journal of The Fourth Military Medical University;2001-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YNAG Xiao-huai1,LI Qing-wen1,ZHANG Jia-jun1,CHENG Ze-nong2(1.Department of Urology,Affiliated Hospital of Medical College,Bengbu 233004;2.Department of Pathology,Bengbu Medical College,Bengbu 233003,China);Expression of Caspase-3 in bladder transitional carcinoma and its significance[J];Journal of Bengbu Medical College;2005-04
2 QIU Xiu Chun 1,3 , YU Cui Juan 1, XU Yan Ming 1, JIA Lin Tao 1, CAO Yun Xin 2, WANG Zhi 1, WANG Cheng Ji 1, FAN Qing Yu 3, YANG An Gang 1 1Department of Biochemistry and Molecular Biology, 2Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710033, China, 3Orthopaedic Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China;Pro-apoptotic effect of a truncated human Bid fusion protein on HeLa cells[J];Journal of The Fourth Military Medical University;2003-21
3 ZHANG Yan Ling 1, CHEN Kang Ning 1, SHAO Shu Qin 1, WANG Ying 2 1Department of Neurology, Southwest Hospital, 2Department of Pathogenic Biology, College of Medicine, Third Military Medcial University, Chongqing 400038, China;Relationship between apoptosis and expression of Caspase-3 after cerebral hemorrhage[J];Journal of The Fourth Military Medical University;2004-01
4 YU Xiao-Lin, LIU Hong, CHEN Wan-Ping, CHENG Peng-YuanOutpatient Department, Fourth Military Medical University, Xi'an 710033, China;Effects of recombinant Caspase-3 on human laryngocarcinoma cell line Hep-2 through coexpression with GFP[J];Journal of The Fourth Military Medical University;2005-21
5 ZHAO Jun-Xia, ZHENG Li-Fen, ZHAO Juan, SHEN Yuan, WANG Yan-Ling, YAN Yun-Li Department of Cell Biology, Basic Medical College, Hebei Medical University, Shijiazhuang, 050017, China;Effects of pholiota nameko polysaccharides on K562 leukemia cell proliferation and Caspase-3 expression in vitro[J];Journal of the Fourth Military Medical University;2007-15
6 WANG xiao-xia1,2,YAO xiao-bao1, JI xian-sheng2, ZHU hong-liang1 1Department of Otolaryngology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China;2Department of Otolaryngology, 451 Hospital of PLA, Xi'an 710054, China;Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis[J];Journal of Southern Medical University;2009-01
7 WU Xue-ling,ZHANG Chun-tao,LIU Chun-yu,et al(National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China);Expression and Antigenicity of Human Granzyme B[J];Chinese Journal of Biologicals;2007-11
8 XU Rui-rong,LU Xin,ZHAO Jun-ming 1(The Affiliated Hospital of Shandong University of TCM,Jinan 250011 1 China Academy of Medical Science,Tianjin 300020);EFFECTS OF LEUKEMIA CELLULAR APOPTOSIS INDUCED BY COMPOUND TONIFYING CHINESE HERBS AND ITS INFLUENCE ON CASPASE-3 EXPRESSION IN LEUKEMIA CELLS[J];Chinese Journal of Traditional Medical Science and Technology;2004-06
9 ZHANG Li hong, JIA Lin tao, BAO Wei, YU Cui juan, ZHAO jing, JIN Ming, WANG Cheng ji, YANG An gang Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi’an 710032, China;Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells[J];Journal of Cellular and Molecular Immunology;2003-02
10 WANG Zhi, ZHAO Jing, ZHANG Li, YU Cui-juan, WANG Cheng-ji, YANG An-gang *Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi’an 710032, China;Establishment of Hela cell line inducibly expressing human active granzyme B[J];Journal of Cellular and Molecular Immunology;2004-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wand Yefu Qi Yipeng Li Zhida Li Xiaofeng Zhu Ying (Institute of Virology, Wuhan University, Hubei, 430072);PCR AMPLIFICATION, IDENTIFICATION OF p35 GENE FROM HaNPV AND ITS CLONING AND EXPRESSION IN PROKARYOTIC CELLS[J];CHINESE JOURNAL OF VIROLOGY;1999-03
2 Miao Ji Tan Wenjie Cong Xu Cong Yu Chen Gang Bi Shengli Zhan Meiyun (Institute of Virology, CAPM, Beijing 100052);THE EXPRESSION AND PROCESSING OF STRUCTURAL POLYPROTEIN OF CHINESE HEPATITIS C VIRUS EXPRESSED IN INSECT CELLS[J];CHINESE JOURNAL OF VIROLOGY;1998-04
3 Shi Xianzong Wang Xunzhang Long Qingxin Wu Wenyan Li Yingqiu Qu Shirui Chen Quhou (State Key Laboratory for Biocontrol, Institute of Entomology Zhongshan University,;STUDIES ON p35 GENE FUNCTION OF TRICHOPLUSIA NI NUCLEAR POLYHEDROSIS VIRUS[J];CHINESE JOURNAL OF VIROLOGY;1999-01
4 CHEN Dan-Ying GAO Yun-Fei LI Li-Xia ZHAI Zhong-He (Life Science College, Peking University, Beijing 100871, China);CHANGES IN KERATIN DURING APOPTOSIS OF PK15 (PORCINE KIDNEY-15) CELLS[J];Acta Zoologica Sinica;2002-01
5 WU Xiao-jian,WU Kai-nan(Department of General Surgery,The First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China);Antiproliferative effect of curcumin on human breast cancer of MCF-7 cells[J];Acta Academiae Medicinae Militaris Tertiae;2006-18
6 GENG Xiao-Ying, WAN Qi, YANG Rong-Jun, WU Song-Di Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China;Effects of hyperthermia on the expressions of skeleton protein and heat shock protein 70 of mouse neurons[J];Journal of the Fourth Military Medical University;2006-12
7 WANG Yu-Ling1,WU Zhen2 (1.College of Basic Medical Sciences,China Medical University,Shenyang 110001,China;2.Hei Long Jiang University,Haerbin 150080,China);Sythesis of curcumin derivatives and their antitumor activities[J];Journal of Guangdong College of Pharmacy;2007-01
8 CHEN Ji da,CAO Wen ling,WANG Yuan liang,CAI Shao xi (Bioengineering Research Institute and SECCS Open Laboratory of Biomechanics and Biorheology,Chongqing University,Chongqing,400044,China);Tissue Engineered Cartilage[J];Foreign Medical Sciences Biomedical Engineering;2001-01
9 LIU Yan,ZHANG Chun-yang,JIANG Hua-mao (Department of Urinary,the First Affiliated Hospital of Liaoning Medical University,Jinzhou 121001 China);Study on the Effects of Curcumin on Proliferation Inhibiting and Cell Cycle of Human Renal Cancer 786-О Cells[J];Journal of Liaoning Medical University;2007-02
10 ZHANG Chuan xi 1, LIN Xin da 1, WU Jun 2 (1.Institute of Applied Entomology, Zhejiang University, Hangzhou 310029,China; 2.Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031,China);Chitinase gene of Helicoverpa armigera nucleopolyhedrovirus and the phylogenetic tree of the baculovirus chitinase genes[J];ACTA ENTOMOLOGICA SINICA;2000-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 Li Xinyou,Wang Jintang,Liu Miao,Wang Quanying,Yang Guangxiao,Li Meng(Department of Orthopedic,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China);The construction of hbmp7 recombinant adenovirus vector[J];Journal of Xi'an Jiaotong University(Medical Sciences);2008-01
2 An Chun-hou, Cheng Yang, Yuan Quan, Li Jian-jun Department of Orthopaedic Surgery, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China;Effects of insulin-like growth factor 1 gene transfection on proliferation of rabbit adipose mesenchymal stem cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-23
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