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《Journal of Chinese Physician》 2004-12
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The Apoptosis Induction Effect of Recombinant Caspase-3 on the Human Osteosarcoma Cell Line SOSP-9901

YU Bing, FAN Qing-yu, YAN Lu,et al. Orthopedics Oncology Institute of PLA, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China  
Objective To investigate the apoptosis induction effect of recombinant caspase-3 expression on osteosarcoma cell line SOSP-9901. Methods Recombinant caspase-3 gene was subcloned into the GFP reporter vector pEGFP-C1 to generate the expression vector pEGFP-caspase-3 by DNA recombinant technique. pEGFP-caspase-3 was transfected into human osteosarcoma cell line SOSP-9901 by Lipofectamine 2000. The expression of recombinant caspase-3 was determined by RT-PCR. The morphological changes of transfected cells were observed under flurescent and electronic microscope. The cell survival rate of transfected cells was assayed by MTT method. Results Recombinant caspase-3 gene could be stably expressed in the transfected SSOP-9901 cells. Recombinant caspase-3 could obviously induce SOSP-9901 apoptosis, and inhibit SSOP-9901 cell proliferation in vitro. Conclusion Recombinant caspase-3 could inhibit the growth of the osteosarcoma cell line SOSP-9901 and induce it into apoptosis.
【Fund】: 国家自然科学基金重点资助项目 (30 330 61 0 )
【CateGory Index】: R738.1
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