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《Journal of Chinese Physician》 2006-11
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The effect of suramin in combination with adriamycin on HIF-1α and VEGF expressions in mice transplanted with lung adenocarcinoma

OU Li-wen,LUO Yun-hai.The Department of Respiratory,the affiliated hospital of XiangNan University.Huo Jianbing,Zhang Ping,The teaching and research unit of clinic nurse,The nurse college of NanHua University  
Objective To study the regulation of Suramin in combination with Adriamycin and its effect on the HIF-1α and VEGF expressions in mice transplanted with lung adenocarcinoma.Methods LA795 cells were subcutaneously inoculated into the mammary fatty pad of T739 mice to establish the lung adenocarcinoma model.After the treatment,40 experimental mice were randomized into four groups(ten in each group).Control group: every mouse was injected into abdominal cavity with 0.2ml 0.9% sodium chloride aqua everyday,16 times total.Adriamycin groups: every mouse was injected into abdominal cavity with sodium chloride aqua(2 mg·kg~(-1)·d~(-1)) on the 4th,11th, and 18th days.Suramin groups: every mouse was injected with Suramin(10 mg·kg~(-1)·d~(-1)) once/day,16 times total.Combination group: the dose is the combination of Adriamycin group and Suramin group.After transplantation,all mice were sacrificed on day 24.The maximum length(a) and the width(b) of the subcutaneous tumors were measured,and the volume of the tumors were calculated.The wet weights of the tumors were also recorded.The specimen of the tumors were fixed and,the HIF-1α and vascular endothelial growth factor(VEGF) expressions in tumor tissues,the subcutaneous tumor microvessel density(MVD) and proliferating cell nuclear antigen(PCNA)were analyzed with immunohistochemistry and imaging system.Results The size and wet weight of the tumors in every treatment groups are significantly lower than those in the control group with the lowest in the combination group.The gray levels of HIF-1α and VEGF in tumor tissues of the suramin group and the combination group are higher than those of the control group.The MVD gray level and the PCNA proliferative index in the suramin group and the combination group are lower than those in control group.The gray levels of HIF-1α and VEGF are the highest in the combination group,but the MVD gray level and the PCNA proliferative index are the lowest in the combination group.The results have statistical significance.The gray levels of HIF-1α and VEGF in Adriamycin group are higher than the control group with no statistical significance.The gray levels of HIF-1α and VEGF in Adriamycin group are significantly lower than those in combination group.The MVD in Adriamycin group is lower than that in control group,but the results are not significantly meaningful.The MVD in Adriamycin group is significantly higher than that in combination group.The PCNA proliferative index in Adriamycin group is significantly lower than that in the control group,and higher than that in combination group.Among the groups,the gray levels of HIF-1α and VEGF have a negative relationship with the MVD and PCNA proliferative index respectively.Conclusion Suramin in combination with Adriamycin may inhibit the upregulation of HIF-1αand VEGF,hence inhibit the formation of new blood vessels in the tumor tissues and the growth of tumor cells.
【Fund】: 湖南省卫生厅科研课题资助项目(C2005033)
【CateGory Index】: R734.2
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1 OU Liwen1,LUO Yunhai1,ZONG Xiaojian2 (1. Department of Respiratory,2. Department of Gynecologic,the Affiliated Hospital of Xiangnan University,Chenzhou,Hunan 423000,P.R.China);Study of Suramin in combination with Adriamycin and its efficacy on the bFGF and NF-КBp65 in the transplantation of Lung-adenocarcinoma of mice[J];China Journal of Modern Medicine;2009-12
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1 ZHANG Ping, HE Jian-Bin, OU Li-Wen, WANG Xiao-HuaDepartment of Respiratory Medicine, The First Affiliated Hospital, Nanhua University, Hengyang, Hunan, 421001,P. R. China;Effects of Suramin in Combination with Cisplatin on Growth and Metastasis of Lung Adenocarcinoma Xenografts in Mice[J];Chinese Journal of Cancer;2006-04
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