CHEMICAL STUDIES ON THE ACTIVE PRINCIPLES OF ANEMARRHENA ASPHODELOIDES EGE
Hong Yongfu(Postgraduate of Phytochemistry
In our experiment, zhimusapgenin, zhimusapogenin A, zhimusaponin B, zhimu-flavone and β-sitosterol were isolated from the fresh rhizoma of XiLinzhimu (Anemarrhena asphcdeloides Bge.)produced in XiLin in Hebei Province and collected in October 1980.They are isolated and purified by means of colum chromatography.The structure of zhimusapogenin is assigned as sarsasapogenin by mp, TLC, chemical identification and spectroscopic analysis (UV,IR and MS)with an authentic sample of sarsasapogenin. Zhimusaponin A is a water-insoluble steroid saponin which was isolated as colorless needle. According to chemical and spectral data, zhimusaponin A is assigned as sarsasapogenin-3-O- β -D-glucosyl-(1-2)-O- β-D-galactopyranoside,i.e.Timosaponin AIII as reported befor.Zhimusaponin B is a water-soluble steroid saponin which was isolated as amorphous powder.By UV, IR, elemental analysis, quantitative analysis of the complete hy-drolytic products and cleaved with emulsin, it is shown that zhimusaponin B may be a new steroid saponin found in this plant and it's composition is zhimusaponin A having another two moles of glucose, and this two moles of glucose are conjoined at zhimusaponin A with β-linkage.The zhimuflavone was identified to be known mangiferin previously obtained from this plant by TLC, PC, UV, IR, PMR and it's chemical properties.β-Sitosterol was isolated from rhizoma Anemarrhenae for the first time, and determined by UV, IR, PMR and MS data.