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《Academic Journal of Second Military Medical University》 1989-01
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MICROASSAY,PREPARATION AND PARTIAL PURIFICATION OF RAT INTERLEUKSN 2

Chen Yijun, et al (Department of Immunology)  
An interleukin 2 (IL-2) microassay based on IL-2 dependent cell line CTLL was established referring to Gillis'method.The data were transformed to probit unit, and the regression line obtained had a good correlation(r0.99).The IL-2 activity of control sample of our laboratory was determined according to recom-binant human IL-2 standard,the ability of IL-2 induced by rat and mouse spleen cells was compared, and the parameters of IL-2 induced by rat spleen cells were detected.The partially purified rat IL-2 was obtained through ammonium sulfate precipitation, gel filtration and chromatography.
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