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Antiendotoxic action of hairy roots of autotetraploid Isatis indigotica Fort.

LI Bo Hua, ZHANG Han Ming, WANG Yin, DING Ru Xian, XU Tie Feng (Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, Shanghai 200433, China)  
Objective:To induce hairy roots from autotetraploid Isatis indigotica Fort.and assess the probability of using hairy roots as medicine instead of normal roots.Methods:Cotyledon leaf explants of autotetraploid Isatis indigotica Fort.were infected by Agrobacterium rhizogenes strain A4.Antiendotoxic action of hairy roots and Banlangen ( Radix Isatidis ) was investigated through pharmacological experiment.Results:Hairy roots of autotetraploid Isatis indigotica Fort.could be induced by Agrobacterium rhizogenes strain A4 and hairy roots had significant antiendotoxic action.Conclusion: Agrobacterium rhizogenes could induce hairy roots from polyploid plants and hairy roots might be a substitute for Chinese tranditional medicine.
【Fund】: 国家自然科学基金! ( 3 9870 919)
【CateGory Index】: R285
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