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《Academic Journal of Second Military Medical University》 2000-08
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Effects of hydroxyl camptothecinum on proliferation and apoptosis of human pancreatic cancer cell line

XU Qing 1, GAO Yong 1, WANG Jie Jun 1, ZHU Chang Li 1, WANG Bing 1, GUO Jing 1, BAO Hong Tao 2 (1.Department of Oncology, Changzheng Hospital, Second Military Medical University, Shanghai 200003,China;2.Department of Medical Affair  
Objective: To study the effect of hydroxyl camptothecinum (HCPT) on proliferation and apoptosis of human pancreatic cancer cell in vitro . Methods: After treated with HCPT at different concentrations (1.562 5, 3.125, 6.25, 12.5 , 25, 50, 100 μg/ml) for 20 24 h, the cultured SW 1990 cell proliferation were evaluated by MTT, and apoptosis by Annexin Ⅴ fluos staining, electronic microscopy, flow cytometry, bcl 2 immunocytochemical staining and in situ TUNEL (TdT mediated dUTP X nick end labeling). Results: (1) The rate of proliferation in each group of 3.125 100 μg/ml was significantly lower than that in the control group ( P 0.01) after treated with HCPT for 24 hours. (2) The apoptosis rate of pancreatic cell was 13.2% and 15.3% after treated with HCPT at the concentration of 50 μg/ml for 20 hours, the rate was 26.1% and 28.4% at 100 μg/ml. (3)Annexin Ⅴ fluos staining and electronic microscopy detection showed the expression of apoptosis was mainly at the early morphological stage at the concentration of 50 μg/ml and the apoptosis rate of early stage was 18.4%. (4) The expression of bcl 2 were less than the control after treated with HCPT 50 μg/ml for 24 hours. Conclusion: HCPT can depress the proliferation of SW 1990 in vitro , partially through the induction of apoptosis, and HCPT is a potential agent for pancreatic cancer chemotherapy. [
【Fund】: 国家自然科学基金!资助项目 (39770 836)
【CateGory Index】: R735.9
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