Study on differentially expressed genes in laryngeal squamous cell carcinoma by cDNA microarray

CHEN Ju Xiang 1, FAN Jing Ping 1, YING Kang 2, LIAO Jian Chun 1, LI Yao 2, TANG Rong 2, ZHOU Shui Miao 3, SUN Ai Hua 1, XIE YI 2, MAO Yu Min 2 (1. Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2. In  

Objective: To screen the differentially expressed genes between laryngeal squamous cell carcinoma and normal laryngeal tissue using cDNA microarray. Methods: The PCR products of 4 096 genes were spotted onto a chemical material coated glass plates in array. DNAs were fixed onto the glass plate after series of treatments. The total RNAs were isolated from the tissues, and were purified to mRNAs by Oligotex. Both mRNAs from the laryngeal squamous cell carcinoma and the normal tissue were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues. Results: Among the 4 096 target genes, 211 356(5.15% 8.87%)genes whose expression level differed in 2 cases and 36 (0.88%) genes differed in 3 cases between the normal and carcinoma tissues in all 4 cases. Conclusion: cDNA microarray technique is effective in screening the differentially expressed genes between 2 different kinds of tissue. Further analysis of those obtained genes will be helpful to understand the molecular mechanism of malignant carcinoma. [

【Key Words】： cDNA microarray laryngeal squamous cell carcinoma gene differential expression
【Fund】： 国家自然科学基金!资助项目 ( 3 9770 792 )
【CateGory Index】： R739.65

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