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《Academic Journal of Second Military Medical University》 2001-05
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Effect of insulin-like growth factor-Ⅰ on osteocalcin secretion of periodontal ligament cells

LIU Hong1, WU Zhi Feng 2, WANG Qin Tao 2, ZHOU Bin 3 (1. Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Periodontology, School of Stomatology, Fourth Military Medical Univers  
Objective: To investigate the effect of insulin like growth factor Ⅰon osteocalcin secretion in periodontal ligament cells. Methods: Human periodontal ligament cells were cultured by tissue explant in vitro , and the concentration of osteocalcin were determined with radio immunological method. Results: Under the condition of L ascorbic acid and β glycerophosphate in culture medium, human periodontal ligament cells secreted osteocalcin time dependently and peaked at the third week; IGF Ⅰ3.125 ng/ml,6.250 ng/ml, 12.500 ng/ml, 25.000 ng/ml could promote the secretion of osteocalcin dose dependently. Conclusion: IGF Ⅰ can increase the secretion of osteocalcin in human periodontal ligament cells.
【Fund】: 国家自然科学基金资助项目 ( 3 970 0 0 3 5 )
【CateGory Index】: R780.2
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