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《Academic Journal of Second Military Medical University》 2002-01
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Expression of c-FLIP mRNA in colorectal cancer cells and its relationship with Fas antigen (CD95) expression

GAO Wen-Chao 1,WANG Yuan-He 1,LI Li 2,WANG Qiang 1,ZHANG Ling-Zhen 2,CAO Lu-Ning 2,DING Er-Xun 1 (1. Department of General Surgery,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Laboratory Diagnosis,Cha  
Objective:To investigate the expression of c-FLIP mRNA in colorectal cancer cells and its relationship with Fas antigen(CD95)expression.Methods:RT-PCR was employed to detect the expression of c-FLIP mRNA and indirect immunofluoresence label flow cytometry was utilized to assay the Fas antigen expression in 3 colorectal cancer cell lines.Results: c-FLIP mRNA was positive in SW1116 and SW620 cell lines and negative in LoVo cell lines.The expression of Fas antigen was much higher in SW1116 and SW620 cell lines than that in LoVo cell lines.Conclusion:The expression of c-FLIP mRNA had positive correlation with the expression of Fas antigen in colorectal cancer cells,and c-FLIP may significantly inhibit the apoptosis induced by Fas in colorectal cancer cells.
【Key Words】： CD95 c-FLIP I-FLICE colorectal neoplasms flow cytometry
【Fund】： 国家自然科学基金资助项目 ( 3990 0 14 3)
【CateGory Index】： R735.34
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