Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Academic Journal of Second Military Medical University》 2003-02
Add to Favorite Get Latest Update

Cell-and time-dependent metallothionein gene expression in testes of rats treated with cadmium

ZHANG Jian Peng, ZHONG Yan, REN Xu Yi,ZHOU Yong, FENG Wei Hua *, JIAO Bing Hua(Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433,China)  
Objective:To compare metallothionein( MT ) gene expression, MT protein accumulation, and cadmium(Cd) retention in freshly isolated testicular various cell types and liver of rats treated with Cd,providing information for the molecular mechnism of testes sensitivity to Cd. Methods: MT 1 and MT 2 mRNA levels were determined by semi quantitative RT PCR analysis followed by densitometry scanning, and MT was estimated by the enzyme linked immunosorbent assay (ELISA). Cd content was determined by atomic absorption spectrophotometry. Testicular lesions were not grossly or histologically observed in rats treated with 4.0 μmol/kg Cd. Results:Both MT mRNA and MT were constitutively present in testes and liver.Testis had higher levels of MT 1 and MT 2 mRNA in 1 h,3 h,6 h compared with control,but MT protein did not increase.Cd exposure also increased hepatic MT s mRNA and MT protein.Conclusion:These results indicate that although Cd exposure results in increase of MT mRNA in testis,it does not enhance MT synthesis,this may account for higher susceptibility of testes to Cd toxicity and carcinogenesis relative to liver.
【Fund】: 国家自然科学基金 (3 9970 63 1)
【CateGory Index】: R363
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 HE Yong-Ji MA Wen-Li WANG Lan WANG Qian ZHANG Xiao-Bo(School of Life Science and Technology,Shanxi University,Taiyuan 030006,China);Metallothionein in Different Tissues of Freshwater Crab Exposed to Cadmium[J];Chinese Journal of Zoology;2007-03
2 Tan Qiong, Zhang Bin;Metallothionein and animal emergency[J];Feed Industry;2007-07
3 LI Lili~1,ZHANG Bin~2,YIN Yulong~1,ZHOU Zhiyang~3,LI Xiaolong~2,LI Tiejun~1(1Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha 410125,China;2College of Animal Science and Technology, Hunan Agriculture University,Changsha 410128,China;3Hunan Gouguang Institute of Medicament Hunan,Changsha 410005,China);Effects of inducement on metallothionein in liver of pig[J];Journal of Guangxi Agricultural and Biological Science;2004-04
4 ZHOU Yan-feng1,YOU Yang1,WU Wei1,YANG Guang2,CHEN Jia-zhang1(1.Freshwater Fisheries Research Center,Key Open Laboratory of Inland Fishery Eco-environment and Resource,Chinese Academy of Fishery Sciences,Wuxi 214081,China;2.Fishery College of Nanjing Agricultural University,Wuxi 214081,China);Dynamic Changes of Metallothioneinn(MT) in Different Tissues of Carassius auratus Exposed to Different Concentrations of Zinc[J];Jiangsu Journal of Agricultural Sciences;2009-04
5 Tan Qiong Zhang Bin;The relationship between metallothionein and biological stress for animal[J];China Feed;2007-13
6 Li Zhang, Jun Cai YeLi Zhang, Jun Cai Ye, Department of Orthopedics and Traumatology, Fujian College of Traditional Chinese Medicine,Fuzhou 350003,Fujian Province,China;Effect of huoxue huayu tang on the expression of vascular endothelial growth factor during early fracture healing[J];Chinese Journal of Clinical Rehabilitation;2004-23
7 BIAN Jian-chun1,GUO Heng-jie1,WANG Han-dong1,LIU Xue-zhong1,YUAN yan1,REN Jian-xin1,LIU Zong-ping1,WANG Zong-yuan1,KANG Shi-liang2,WU Rui3 (1College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,Jiangsu;2College of Veterinary Medicine,Northeast Agricultural University,Haerbin 150030;3 College of Veterinary Medicine,Heilongjiang August First Land Reclaimation University,Daqing 163319,Heilongjiang);Isolation and Comparison of Low-Molecular-Weight Metal Binding Protein in Testis and Liver of Rabbits Treated with Cadmium[J];Scientia Agricultura Sinica;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIN Zhong ning, DONG Sheng zhang, CAI Ying, DONG Shu yun, YU Gui ying, REN Tie ling (Department of Environmental Health, Sun Yat sen University of Medical Sciences, Guangzhou 510089, China);IN VITRO STUDY ON THE DNA INJURIES AND REPAIR OF SPLENIC LYMPHOCYTE INDUCED BY CADMIUM-METALLOTHIONEIN[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-02
2 Ying Guishuang,Huang Xingshu Toxicology Department,Zhejiang Medical Univ.Hangzhou 310031;THE TESTICULAR TOXICITY ASSAY OF DIETHYLSTIBESTROL,ENDOSULFAN AND CADMIUM CHLORIDE WITH THE PRIMARY CULTURE OF RAT SERTOLI CELLS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1997-01
3 GUA Jun-hua XU Zhuo-li SONG San-tai LIU Qin-di WU De-zheng(Centre of Cancer Research, North Taiping Road Hospital, Beijing. 100039);PROTECTIVE EFFECT. OF METALLOTHIONEIN INDUCING METALS ON LETHAL TOXICITY OF CISPLATIN IN MICE[J];Chinese Journal of Cancer;1993-06
4 WANG Jun1,WU Ji-song1,LI Cai-yan1,XIE Chang1,ZHANG Yuan-yuan1,WU Wei1,2(1 Fishery College,Nanjing Agriculture University,Wuxi 214081,Jiangsu,China ;2 Freshwater Fisheries Research Center,Key Open Laboratory of Inland Fishery Eco-environment and Resource,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China);On acute toxicity of deltamethrin on Pseudorasbora parva under zinc ion stress[J];Journal of Safety and Environment;2007-04
5 ZHOU Yan-feng1,CHEN Jia-zhang1,2,YANG Guang1,YANG Lin1,WU Wei1,2(1 Fishery College,Nanjing Agricultural University,Wuxi 214081,Jiangsu,China ; 2 Key Open Laboratory of Inland Fishery Eco-environment and Resource,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China);On the dynamic variations of metallothionein in the different tissues of Carassius auratus due to the exposure of different concentrations of cadmium[J];Journal of Safety and Environment;2008-03
6 WANG Xiang 1, ZHANG Da cheng 1,LI Ting 1, WANG Wei 1, Ren Hong wei 2,Han Tie gang 2,Yu Mei min 2 (1. Institute of Microelectronics, Peking University, Beijing 100871,China; 2. School of Life Science, Peking University, Beijing 100871,China);Development of the purification for metallothionein (MT)[J];Micronanoelectronic Technology;2003-Z1
7 LI LING-YUAN PAN AI-HuA RUBING-GEN SHENTONG(Department of Biology, Peking University, 100871)TIE FANG WANG WEN-QING(Department of Technical Physics, Pelting University, 100871);PURIFICATION AND CHARACTERIZATION OF ZN-METALLOTHIONEINS FROM CADMIUM-INDUCED RABBIT LIVER[J];Acta Zoologica Sinica;1993-03
8 WANG Lan YANG Xiu-Qing WANG Qian WANG Ding-Xing (Life Science,Shanxi University,Taiyuan 030006,China);THE ACCUMULATION OF Cd~(2+) AND THE EFFECT ON EST IN FIVE TISSUES AND ORGANS OF ERIOCHEIR SINENSIS[J];Acta Zoologica Sinica;2001-S1
9 LIN Zhi-Lan ① LI Fu-Rong ② MA Guo-Dong ① LIANG Shuang ① (①College of Life Sciences, Peking University, Beijing 100871, China) (②Department of Biology, Xinyang Teachers College, Xinyang 464000, Henan, China);ISOLATION, PURIFICATION AND IDENTIFICATION OF METALLOTHIONEIN IN CHINESE SOFT-SHELLED TURTLE (PELODISCUS SINENSIS) LIVER[J];Acta Zoologica Sinica;2002-01
10 WANG Lan ①** SUN Hai Feng ② LI Chun Yuan ③ (① College of Life Science and Technology, Shanxi University, Taiyuan 030006, Shanxi, China) (② Collleg of Chemistry Engineering, Shanxi University, Taiyuan 030006, Shanxi, China) (③ Ch;EFFECTS OF CADMIUM ON SPERMATOGENESIS IN FRESHWATER CRAB (SINOPOTAMON YANGTSEKIENSE)~*[J];Acta Zoologica Sinica;2002-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Bin1,XIAO Ding-fu1,LI Li-li2,CHEN Yu-guang1,WANG Ying1,WU Zong-ming1,ZHANG Fu-sheng2,LAN Xin-yi1(1.College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;2.Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China);Studies on metabolism orderliness of metallothionein in male calves from Chinese Holstein[J];Acta Prataculturae Sinica;2008-06
2 LU Hao1,LIU Zong-ping2,ZHAO Bao-yu1(1.College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China;2.College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu,225009,China);Progress on Biological Functions of Metallothionein[J];Progress in Veterinary Medicine;2009-01
3 Tan Qiong, Zhang Bin;Metallothionein and animal emergency[J];Feed Industry;2007-07
4 ZHANG Bin1,TAN Qiong1,XIAO Bing-nan3,LI Li-li2,CHEN Yu-guang1,XIAO Ding-fu1,LIU Hai-lin3,WU Zong-ming1,ZHANG Fu-sheng2(1.College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;2.Institute of Subtropical Agricultural Ecology,The Chinese Academy of Sciences,Changsha 410125,China;3.Institute of Animal Science and Veterinary of Hunan Province,Changsha 410131,China);Effects of exogenous metallothionein on milkability and anti-oxidative status in dairy cattle[J];Acta Prataculturae Sinica;2009-06
5 LIU Yun-hua~1, ZHANG Bin~1, LI Li-li~2 (1.College of Animal Science and Technology, Hunan Agriculture University,  Changsha Hunan 410128, China; 2.Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha Hunan 410125, China);The Application and Perspective Metallothionein in Farming Industry[J];Ecology of Domestic Animal;2004-04
6 WU Li-zhuan(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025,China)LI Li-li(Institude of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410128,China)ZHANG Bin(College of Animal Science,Hunan Agricultural University,Changsha,Hunan 410128,China);The Application of Metallothionein in Animal Nutrition[J];Journal of Yangtze University(Natural Science Edition);2006-02
7 Wan Hong Xiong Chengliang Qin Ying The Family Planning Institute of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030;The Empirical Study of in Vitro Transfection by Chemical Synthesized siRNA in Rat Sertoli Cells[J];Chinese Journal of Family Planning;2008-08
8 CAO Xing-wu Department of Andrology, China-Japan Friendship Hospital, Beijing 100029, China;Progress in the Research of Sertoli Cell Cytoskeleton of the Testis[J];National Journal of Andrology;2008-08
9 ZHANG Zhi-qu,ZHAN Ping. Department of Environment Hygiene,School of Public Heath,Chongqing Medical University,Chongqing 400016,China;Effects of Bisphenol A on Rat Sertoli Cell Function and Its Mechanism[J];Journal of Environment and Health;2009-12
10 ZHOU Yue1,CHEN Yao2,ZHANG Peng-guo3,et al(1.Department of Biology Medical Engineering,Pharmaceutic Cllege,Jilin University,Changchun 130021,China;2.Centre of Medicine and Food Reevaluation,Jilin University,Changchun 130021,China;3.The Second Hospital of Jilin University,Changchun 130041,China);Experimental study of Xugui capsule in the treatment of fracture[J];Jilin Medical Journal;2010-13
China Proceedings of conference Full-text Database 2 Hits
1 XIAO Ding-fu1 ZHANG Bin1 LI Zhong-yi1 LI Li-li2 CHEN Yu-guang1 PENG Hui-zhen1(1 College of Animal Science and Technology,Hunan Agricultural University,ChangSha 410128,China;2 Institute of Subtropical Agriculture,CAS,ChangSha 410125,China);Effects of Different Stress Factors on Metallothionein Synthesis in Mouse Liver[A];[C];2008
2 Zhang Bin1,Xiao Ding-fu1,Li Li-li2,Chen Yu-guang1,Wang Yin1,Wu Zong-ming1,Zhang Fusheng2,Lan Xin-yi1(1 College of Animal Science and Technology,Hunan Agricultural University,Changsha,PRC,410128 2.Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,PRC,410125);Studies on metabolism orderliness of metallothionein in dairy cattle[A];[C];2008
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved