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《Academic Journal of Second Military Medical University》 2003-02
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Establishing inbred strain of coagulation factor Ⅸ-knockout mouse

HAO Guang Rong 1* , SUN Wei 1, HU Wei Jiang 1, CHEN Li 2, CUI Shu Fang 1, TANG Qiu 1, CAO Ping 1 (1. Laboratory Animal Center,Second Military Medical University,Shanghai 200433,China; 2. Institute of Genetics Research,Fudan University)  
Objective:To establish a inbred strain of the coagulation factor Ⅸ knockout mice with stable heredity and obvious clinical symptoms. Methods:The genomic DNA from tail tissue of mice were analyzed by PCR. F Ⅸ activity plasma prothrombin time and kadin partial thromboplastin time were measured by one stage method.The positive mice were mated with brother sister by optimum seeking method.The stocks of every generation were selected from first born mice.The homogeneous mice were selected after F 8. Results: PCR analysis of F Ⅸ mice(F 8) was 92% positive and F Ⅸ mice(F 12 ) was 100% positive. F Ⅸ activity (2.47±1.75)% was lower than normal mice (148.18±46.98)%. F Ⅸ knockout mice were developed 12th generation and there were spontaneous bleeding in the factor Ⅸ knockout mice. Conclusion:The result showed that the coagulation F Ⅸ knockout mice inbred strain were established and exhibited a phenotype similar to human hemophilia B.
【Fund】: 上海市科学技术发展基金 (9949190 3 2 )
【CateGory Index】: R554.1
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1 Lai Lihui, Chen Li, Zhou Hong 1,Wang Jianmin 1,Hao Guangrong 2,Lu Daru,Sun Wei 2, Zhou Caihong,Qiu Xinfang, Xue Jinglun (Institute of Genetics, Fudan University; Changhai Hospital, Second Military Medical University 1; Animal Center, Second Milit;Studies on the Phenotype and Genetic Stability of Coagulation Factor Ⅸ-deficient Mouse Model for Human Hemophilia B[J];JOURNAL OF FUDAN UNIVERSITY;1999-04
Chinese Journal Full-text Database 2 Hits
1 LU Huazhong , CHEN Li WANG HongweiWU Zhijian WU Xiaobing WANG XuefengWANG Hongli LU Daru QIU Xinfang& XUE JinglunThe State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China;The State Key Laboratory of Molecular Virology and Gene Engineering, Chinese Academy of Preventive Medical Sciences, Beijing 100050, China;Shanghai Institute of Hematology, Ruijin Hospital of Shanghai Second Medical University, Shanghai 200025, ChinaPresent address: Laboratory for Blood Engineering, Shanghai Institute of Blood Transfusion, Shanghai Blood Center, Shanghai 200051.;Gene therapy for hemophilia B mediated by recombinant adeno-associated viral vector with hFIXR338A, a high catalytic activity mutation of human coagulation factor IX[J];中国科学(C辑:生命科学)(英文版);2001-06
2 CHEN Li, CHEN Haoming, LI Ge, LU Daru & XUE Jinglun State Key Laboratory of Genetic Engineering, Institute of Genetics,School of Life Sciences, Fudan University, Shanghai 200433, China Correspondence should be addressed to Xue Jinglun (e-mail: jlxue@ fudan.ac.cn);Novel rAAV production system with low contamina-tion of helper virus[J];科学通报(英文版);2003-05
【Secondary Citations】
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1 Dai Xuming, Xue Hong, Yang Hua, Hu Yiping, Fu Jiliang (Open Laboratory of Medical Molecular Genetics, Department of Basic Medicine, Second Military Medical University, Shanghai, 200433);Construction and application of gene targeting replacement vector[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-01
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