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《Academic Journal of Second Military Medical University》 2003-04
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Inhibition effects of three kinds of bufotoxins on human SMMC-7721 and BEL-7402 hepatoma cells lines

SU Yong-Hua, YIN Xi-Cai, XIE Jue-Min, GAO Bo, LING Chang-Quan (1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University .Shanghai 200433, China; 2. Department of Research and Development ,Anhui Jinchan Drug Limited Coporation)  
Objective: To study the effect and mechanism of bufalin, cinobufagin and resibufogenin on human hepatoma cell lines. Methods: SMMC-7721 and BEL-7402 cells were incubated with the above 3 drugs at different concentration separately. Mitomycin C was used as control drugs. The inhibitory rate was analyzed by MTT and trypan-blue staining assay. Cell DNA contents and apoptosis were analyzed by flow cytometer. Results: Bufalin and cinobufagin even at low concentration remarkably inhibited the cells growth, stopped the cells in G2/M phase and induced the cells apoptosis. High dose of bufalin and cinobufagin damaged the cells membrane effectively. Anti-tumor effects of both bufalin and cinobufagin were stronger than that of mitomycin C at the same concentration, and the effect of bufalin was stronger than that of cinobufagin. Conclusion: Bufalin and cinobufagin can efficiently depress the cancer cells growth, inducing cells apoptosis and demolishing cells membrane are 2 ways of their anti-tumor effects, while resibufogenin has weak anti-tumor effect.
【Fund】: 国家自然科学基金青年科学基金(30200364).
【CateGory Index】: R735.7
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