Effect of lipopolysaccharides on expression of cholecystokinin receptor mRNA in rat pulmonary interstitial macrophages
XU Shun-Jiang,GAO Wei-Juan ,YAO Yu-Xia,GU Zhen-Yong ,CONG Bin (. Department of Pathophysiology,Hebei Medical University,Shijiazhuang 05007,China; 2. Hebei Center of Disease Control)
Objective: To investigate the expression of cholecystokinin (CCK) receptor mRNA and the effect of lipopolysaccharides(LPS) on its expression in rat pulmonary interstitial macrophages (PIMs). Methods: The PIMs isolated from rat lung tissues were purified by the collagenase digestion method combined with alveolar lavage and pulmonary vessel perfusion, and incubated with LPSQO mg/L) for 0. 5-12 h. The subtypes of CCK receptor and the effects of LPS on its expression were investigated by RT-PCR and Southern blot analysis in rat PIMs. Results: CCK-AR and CCK-BR mRNA were detected in rat PIMs by means of RT-PCR; their amplification products were approximately 1. 37 kb and 480 bp,respectively. The expression of CCK-BR mRNA was higher than that of CCK-AR mRNA,and the expression of CCK-AR and CCK-BR mRNA could be up-regulated obviously by LPS incubation for 2 to 12 h. r-32P-ATP 5'-end-labelled probes showed specific hybridization bands by Southern blot analysis of CCK-AR and CCK-BR mRNA products. Conclusion: There are CCK-AR and CCK-BR mRNA expressions in rat PIMs and the expressions can be up-regulated by LPS.