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《Academic Journal of Second Military Medical University》 2003-12
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Determination of tenacissosides J in Marsdenia tenacissima by capillary zone electrophoresis

XING Wang-Xing,CHENG Rong-Zhen,LIN Pei-Ying ,CHEN Bin ,MI He-Ming1,WU Yu-Tian" (1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, Chinas 2. Department of Pharmaceutical Preparations, No. 117 Hospital of PLA, Hangzhou 310013; 3. Hangzhou Jianfeng Dekang Pharmaceutical Limited Company)  
Objective; To determine the contents of tenacissosides J in Marsdenia tenacissima by capillary zone elec-trophoresis(CZE). Methods:Using benzoic acid as the internal standard (IS), the electrophoretic separation was achieved with 20% acetonitrile in 50 mmol/L borate buffer (pH 9. 28) as the running buffer and a voltage of 20 kV was applied to the 47 cm× 75 μm (ID) capillary. The analysis were introduced into capillary by siphon (5 s) and determined with on-column UV monitoring at 214 nm. Results: The determination could be completed within 15 min and the analytical results of samples demonstrated a good linear relationship within 43. 43-1 732. 06 μg/ml. The relative standard deviation (RSD) of intra-day was less than 2. 50% and that of inter-day was less 5. 16%. The recoveries were 98. 1%-104. 7%. Conclusion:CZE is accurate, simple and reliable,which can be applied to determine tenacissosides J in Marsdenia tenacissima.
【Fund】: 全军医学科研重点课题基金(01Z064)
【CateGory Index】: R284
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