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《Academic Journal of Second Military Medical University》 2004-05
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Studies on in vitro antitumor activities of a new sterol HEO from sea hare

LIN Hou-Wen , GAO Yong, LIU Gao-Lin , WANG Jie-Jun, ZHANG Chun( Department of Pharmacy, Changzheng Hospital t Second Military Medical University, Shanghai 200003, China; Department of Oncology, Changzheng Hospital)  
Objective:To study the in vitro antitumor activities of a new sterol HEO from sea hare. Methods : With 3H-TdR incorporation experiment, Annexin V fluorescence dye, flow cytometry, transmission electron microscope,human myel-ogenous leukemia cells (HL-60) cultured in vitro were tested to observe the effect of HEO on cell cycle and in inducing apop-tosis. Results:The IC50 of HEO to HL-60 in 24 h was 1. 4 mg/L. HEO obviously inhibited the logarithm growth of HL-60 at the concentration of 0. 8 mg/L. The experiment of 3H-TdR incorporation showed that the inhibitory effect on proliferation of HL-60 cells was apparently enhanced as the time of HEO action prolonged. Annexin V fluorescence dye showed that after 24 h HEO action,a large number of HL-60 cells became apoptotic. Transmission electron microscopy revealed that the chromatin of tumor cells presented with apoptosis features. The analysis of cell cycle showed that the number of G2 + M cells were increased. Conclusion :HEO can inhibit the growth of HL-60 and kill the tumor cells by inducing apoptosis. The influence on the cell cycle indicates that antitumor activities of HEO may result from M phase block.
【Fund】: 国家自然科学基金(30000219) 中国科学院上海有机化学研究所生命有机国家重点实验室开放课题(20010710).
【CateGory Index】: R284
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Chinese Journal Full-text Database 2 Hits
1 MA Yu1,ZHANG Junxia2(1.Department of Secondary Technical and Vocation,Chengdu University,Chengdu 610015,China;2.School of Medicine and Nursing,Chengdu University,Chengdu 610015,China);Research Progress of Cancer-fighting Marine Animals[J];Journal of Chengdu University(Natural Science Edition);2009-03
2 HUANG Fang-fang,YANG Yong-fang,DING Guo-fang School of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan 316004,China;Advances in the study of antitumor activities of the marine mollusks extracts[J];Journal of China Pharmaceutical University;2009-03
China Proceedings of conference Full-text Database 1 Hits
1 ;无花果残渣中抗肿瘤成分提取技术[A];[C];2006
Chinese Journal Full-text Database 2 Hits
1 XU Qing 1, GAO Yong 1, WANG Jie Jun 1, ZHU Chang Li 1, WANG Bing 1, GUO Jing 1, BAO Hong Tao 2 (1.Department of Oncology, Changzheng Hospital, Second Military Medical University, Shanghai 200003,China;2.Department of Medical Affair;Effects of hydroxyl camptothecinum on proliferation and apoptosis of human pancreatic cancer cell line[J];Academic Journal of Second Military Medical University;2000-08
2 LIN Hou Wen 1,TANG Hai Feng 2,LIU Gao Lin 1,ZHANG Chun 1,SHEN Yang 1,YI Yang Hua 2,WU Hou Ming 3 (1.Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China; 2.Research Center for Marine Drugs,College;Studies on antineoplastic constituents from marine mollusc sea hare (Ⅱ)[J];Academic Journal of Second Military Medical University;2002-03
Chinese Journal Full-text Database 3 Hits
1 Zhang Cheng hui, Ren Zhonghai, Li Changsheng. Oncology Department, Nan Yang Center Hospital, Henan Province, 473000, China;Recent effect of combination chemotherapy of hydroycamptothecin and 5-FU、 MMC for advanced pancreatic cancer.[J];Guangzhou Medical Journal;2003-05
2 YANG Feng-ke,ZHANG Zhan-tao(College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China);The Research Development of Biological Activity of Imidazole Alkaloid of Marine Natural Products[J];Chemistry & Bioengineering;2006-05
3 FANG Cai-wang~(1,2),HUANG He-qing~(1,2*),YAN Li~1,CAI Zong-wei~(2,3)(1.School of Life Science,2.State Key Laboratory of Physical Chemistry of Solid Surface,College Chemistry & Chemical Engineering,Xiamen Univ.,Xiamen 361005,China;3.Department of Chemistry,Hong Kong Paptist Univ.,Kowloon Tong,Hong Kong,China);Characteristic Study of Egg Laying Hormone and Its Decomposed Produces from Aplysia Egg with MALDI-TOF Mass Spectrometry Technology[J];Journal of Xiamen University(Natural Science);2006-05
Chinese Journal Full-text Database 10 Hits
1 WANG Yi-Xian ZHANG Xiang-Lian GAO Ling ZHANG Qin-FenKANG Shen-Hai(Jiangsu Institute of Cancer Research, Nanjing 210009) WANG Yi-Lin MA Kai JIANG Wei-Bing WANG Liang-Ju(Nanjing Agronomic University);ANTITUMOR ACTIVITY OF FICUS[J];Chinese Journal of Cancer;1990-03
2 Yang Xinlin Zhu Hesun (Center for Research on Materials Science,Beijing Institute of Technology,Beijing 100081) Xu Jianlan Kuang Qun (China Wuxi Sanlian Advanced Science & Technology Development Co.Ltd,Wuxi 214021);Antitumor Activity of the Ganoderma Lucidum Spore Alcohol Extract in Vitro[J];北京理工大学学报(英文版);1997-04
3 GU Zhong min,MA Zhong sen,AN Ji hong, LI Bo △ (Dept.of Respiration ,The Second Hospital,Jilin University,Changchun 130041,China);Determination of NK Cytotoxicity by Improved MTT Assay[J];Journal of Norman Bethune University of Medical Science;2003-01
4 TU Peng-Fei △, GUO Hong-Zhu,GUO De-An [Peking University Modern Research Center for Traditional Chinese Medicine (P KUMRCTCM), Beijing 100083,China];Researches on active constituents of natural and traditional medicine and develo pment of new drugs[J];Journal of Beijing Medical University;2002-05
5 CHEN Xin, WU Chen yi. (Department of General Surgery,the First Affiliated Hospital,Chongqing University of Medical Sciences, Chongqing 400016, China);Effect of arsenic trioxide on angiogenesis of a heterologous graft model for human breast infiltrating duct carcinoma in nude mice[J];Chongqing Medical Journal;2003-06
6 XU Hong-li~(1),GUO Ting-ting~(2),GUO Yi-feng~(1),ZHANG Jian-peng~(1),FENG Wei-hua~(1*),JIAO Bing-hua~(1*)(1.Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China;2.Central Laboratory,Aqua-life Science and Technology College,Shanghai Fisheries University,Shanghai 200090);Isolation and purification of water-soluble polysaccharide MP-Ⅰ from Mytilus coruscus and study on its in vitro anti-tumor activity[J];Academic Journal of Second Military Medical University;2006-09
7 SHANG Peng,YANG Tie hong, JIA Min, MEI Qi bing, ZHU De sheng, ZHAO Wen ming,ZHAO De hua (Institute of Bioengineering, Xi'an Jiaotong University, Xi'an 710049, China);Inhibitory effects of polysaccharides of Angelica sinensis (Oliv.)Diels on murine transplanted tumors[J];Acta Academiae Medicine Militaris Tertiae;2001-11
8 YAN Xiao-Yu, SHI Jian-Guo, YAN Qing-Guo, CHEN Sheng-Quan, ZHANG Zhi-Pei, LI Qin-Long, ZHANG JingDepartment of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Combination of arsenic trioxide and ascorbic acid inhibits proliferation and induces apoptosis in human hepatoma cell line[J];Journal of The Fourth Military Medical University;2005-11
9 Zhang Erxian and Yu Lijun (Department of Biochemistry ,Science Faculty ,Shantou University ,Shantou 515063);STUDIES ON POLYSACCHARIDE FROM SARGASSUM THUN-BERGII FOR ITS ABILITY TO SCAVENGE ACTIVE OXYGEN SPECIES Ⅱ On Effect of Far Ultraviolet Radiation on Antioxidation of Polysaccharides[J];Chinese Journal of Marine Drugs;1997-03
10 Gu Qianqun,Fang Yuchun,Wang Changyun et al (Marine Drugs and Foods Institute,Ocean University of Qingdao,Qingdao 266003);STUDIES ON THE CHEMICAL COMPOSITION AND AN-TITUMOR ACTIVITY OF THE GLYCOPROTEIN FROM CHLAMYS (AZUMAPECTEN)FARRERI[J];Chinese Journal of Marine Drugs;1998-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 LIN Hou wen, ZHANG Chun, YI Yang hua, et al (Department of Pharmacy, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003);Studies on antineoplastic constituents from chinese marine mollusc sea hare(Ⅰ)[J];Chinese Journal of Marine Drugs;2001-03
2 Li Deling;Wang Huixin;Zhou Tingchong (Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850,China).;Methods for Detecting Programmed Cell Death.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1996-04
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