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《Academic Journal of Second Military Medical University》 2004-05
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Studies on in vitro antitumor activities of a new sterol HEO from sea hare

LIN Hou-Wen , GAO Yong, LIU Gao-Lin , WANG Jie-Jun, ZHANG Chun( Department of Pharmacy, Changzheng Hospital t Second Military Medical University, Shanghai 200003, China; Department of Oncology, Changzheng Hospital)  
Objective:To study the in vitro antitumor activities of a new sterol HEO from sea hare. Methods : With 3H-TdR incorporation experiment, Annexin V fluorescence dye, flow cytometry, transmission electron microscope,human myel-ogenous leukemia cells (HL-60) cultured in vitro were tested to observe the effect of HEO on cell cycle and in inducing apop-tosis. Results:The IC50 of HEO to HL-60 in 24 h was 1. 4 mg/L. HEO obviously inhibited the logarithm growth of HL-60 at the concentration of 0. 8 mg/L. The experiment of 3H-TdR incorporation showed that the inhibitory effect on proliferation of HL-60 cells was apparently enhanced as the time of HEO action prolonged. Annexin V fluorescence dye showed that after 24 h HEO action,a large number of HL-60 cells became apoptotic. Transmission electron microscopy revealed that the chromatin of tumor cells presented with apoptosis features. The analysis of cell cycle showed that the number of G2 + M cells were increased. Conclusion :HEO can inhibit the growth of HL-60 and kill the tumor cells by inducing apoptosis. The influence on the cell cycle indicates that antitumor activities of HEO may result from M phase block.
【Fund】: 国家自然科学基金(30000219) 中国科学院上海有机化学研究所生命有机国家重点实验室开放课题(20010710).
【CateGory Index】: R284
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