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《Academic Journal of Second Military Medical University》 2005-10
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Effects of intratumoral injection of telomerase inhibitor AZT on implanted liver cancer in rats

ZHANG Huo-jun, TIAN Jian-ming~*, WANG Pei-jun, SHAO Cheng-wei, YANG Ji-jin, ZUO Chang-jing, SHENG Jing, HAO Qiang, YUAN Min (Department of Radiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)  
Objective:To observe the effects of intratumoral injection of telomerase inhibitor (azidothymidine, AZT) on implanted liver cancer in rats so as to assess the therapeutic value of AZT for malignant tumor. Methods: Liver cancer models were developed with male adult SD rats by injecting cancerous suspension (Walker-256) into the rat liver under the guidance of B ultrasound. Male Wistar rats were used for tumor passaging. Forty-two tumor model rats were equally randomized into AZT group and control group: intratumoral injection of AZT was given to AZT group and equal volume of normal saline was given to control group. The telomerase activity,apoptotic indices(AI) and tumor volumes were determined in 2 groups. Telomerase activity was examined by telomeric repeat amplification proctocol coupled with ELISA (TRAP-ELISA). Morphological changes of apoptotic tumor cells were observed with MG-P-MY staining and AI was calculated. Tumor volumes were measured 6 d after treatment in 2 groups. Results: The tumor volume was significantly reduced in AZT group compared with that in control group 6 d after treatment ( mm~3 vs mm~3, P0.05); the telomerase activity was significantly lower in AZT group compared with that in control group(0.426±0.162 vs 0.767±0.10,P0.05); and the AI was significantly higher in AZT group compared with that in control group ( % vs %, P0.05) .Conclusion: AZT can decrease telomerase activity, induce tumor cell apoptosis and slow down tumor growth, indicating that AZT might be a feasible treatment for malignant tumors.
【Fund】: 国家自然科学基金(30020233);; 全军医药科研“十五”面上项目基金(01M32018)
【CateGory Index】: R735.7;
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