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Preparation,identification and immunological activities of polysaccharides extracted from Mytilus coruscus

WANG Jun,XU Hong-li,ZHANG Jian-peng,LIU Jun-hua,FENG Wei-hua~(*),JIAO Bing-hua~(*) (Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China)  
Objective:To isolate and purify active polysaccharides from Mytilus coruscus(MPs) and to determine their purities and monosaccharide components.Methods: The homogenization of Mytilus coruscus was extracted by boiling water,followed by protein removal(Sevage method),alcohol fractional precipitation,chromatography with DEAE-cellulose 52,Sephacryl S-200 column and HPLC.The sugar concentrations in MPs were determined by λ_(max) of full wave-length scan and sulphuric acid-phenol colorimetry.IR chromatogram,UV absorption spectrum,staining-combined colorimetry and HPLC were separately applied to determine the homogeneity of the obtained MPs;GC-MS was used to analyze their monosaccharide components.The immunological activities of purified MPs were tested.Results: Eight components,MPs-A1/A2/B1/B2/B3/C1/C2/C3,were extracted from Mytilus coruscus.~(1)HNMR indicated that MPs-B1,with a molecular weight of 3 510 000,was an α-hexapyranose,with glucose and galactose(11) as its major components.Immunological tests showed that MPs-B1 promoted the activities of NK cells,peritoneal marcophages and plaque-forming cells.Conclusion: With modified preparation process,the MPs in this paper are obviously different from those obtained from other similar studies in physiochemical properties,sugar contents and monosaccharide components.MPs-B1 has a positive regulatory immunological function.
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