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《Academic Journal of Second Military Medical University》 2006-01
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Primary active components in sepia: a qualitative and quantitative analysis

JIANG Yun-yun~(1),YE Guang-ming~(2),FAN Guo-rong~(1*)(1.Shanghai Key Laboratory for Pharmaceutical Metabolites Research/Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.Department of Pharmaceutics,No.102 Hospital of PLA,Changzhou 213003)  
Objective:To establish a method for qualitative and quantitative analysis of the major active components in sepia.Methods: Melanin was identified by dissoving it in a basic solution to see whether there was segregation of a black substance.Glycine,asparagines,glutamic acid and alanine in sepia were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer.Results: Melanin in sepia was dissolved in 2 mol/L NaOH solution and black substance was obtained in 2 mol/L HCl solution.The recoveries of the above 4 amino acids and iron were within the range of 95%-105% with RSD5%(n=6).The mean contents of glycine,asparagines,glutamic acid,alanine and iron in 3 batches of sepia samples were 7.37,11.23,12.05,7.32 and 0.206 mg·g~(-1),respectively.Conclusion: The method in this article is feasible for quantitative and qualitative analysis of bioactive contents in sepia;it has good repeatibility and may be used for the quality control of sepia.
【CateGory Index】: R284
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