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《Academic Journal of Second Military Medical University》 2006-09
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Isolation and purification of water-soluble polysaccharide MP-Ⅰ from Mytilus coruscus and study on its in vitro anti-tumor activity

XU Hong-li~(1),GUO Ting-ting~(2),GUO Yi-feng~(1),ZHANG Jian-peng~(1),FENG Wei-hua~(1*),JIAO Bing-hua~(1*)(1.Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China;2.Central Laboratory,Aqua-life Science and Technology College,Shanghai Fisheries University,Shanghai 200090)  
Objective:To separate and purify polysaccharide MP-Ⅰ from Mytilus coruscus and to investigate its in vitro anti-tumor activity.Methods: The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method.The polysaccharide MP-Ⅰ was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography.Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography.The in vitro anti-tumor activity of MP-Ⅰwas assessed using MTT method.Results: The yield of MP-Ⅰwas 2.14%.MP-Ⅰ is mainly constituted by glucose.MP-Ⅰ(at 0.5,0.1,0.02 mg/ml) had different degrees of inhibitory effects on HO-8910,MCF-7, K562,and SMMC-7721 tumor cells in vitro(P0.01).The inhibitory rates of MP-Ⅰat 0.5 mg/ml against HO-8910 and MCF-7 cells were 30.55% and 36.38%,respectively.Conclusion: Polysaccharide MP-Ⅰ is mainly constituted by glucose and it has inhibitory effect on tumor cell lines HO-8910,MCF-7,K562,and SMMC-7721 in vitro.
【Fund】: 国家高技术研究发展计划/863计划(2002AA2Z3325);; 浙江省科技计划项目(2005ZJKJJH2202)~~
【CateGory Index】: R284;R285
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