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Drug and protein interactions:progress of research methods

JIANG Hong~(1,2) AN Pu-li~1 JIANG Ye~(1*) 1.Department of Analytical Chemistry,School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China; 2.Equipment Center of Department of Training,Bethune Military Medical College,Shijiazhuang 050081  
Interaction between drug and protein is a major research field of life science.This article reviews the methods used in related research in the past 5 years,including spectroscopy,equilibrium dialysis,ultrafiltration,microdialysis method, capillary electrophoresis,NMR,X-ray diffraction,electrochemical,affinity chromatography,HPFA,HPLC,etc.It has been showed that combined usages of several technologies are always needed to elucidate an interaction between drug and protein.
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