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《Academic Journal of Second Military Medical University》 2009-07
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HPLC-TOF/MS in identification of chemical components in Ginseng

ZHUANG Jiang-neng1,ZHANG Hai2,LIU Xiao-fan3,ZHAO Liang2,ZHU Zhen-yu3,ZHANG Guo-qing2,CHAI Yi-feng31.Department of Medical Adminstration,No.62 Hospital of PLA,Puer 665000,China2.Department of Pharmacy,Eastern Hepatobiliary Hospital,Second Military Medical University,Shanghai 2004383.Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433  
Objective:To identify the chemical components in traditional Chinese herbal medicine Ginseng by high performance liquid chromatography diode array detector (HPLC-DAD) and High performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS).Methods: An Agilent Zorbax XDB-Cl8 column (250 mm×4.6 mm,5 μm) was used for isolation and identification of chemical components in Ginseng with a mobile phase of acetonitrile and water in gradient elution.A gradient program was used as follows:1-35 min,19% A; 35-55 min,19%-29% A; 55-70 min,29% A; 70-110 min,29%-40% A; 110-150 min,95% A.The flow rate was set at 1.0 ml/min,the injection volume was 20 μl,and the column temperature was set at 20℃.The time-of-flight mass spectrometer was equipped with an EIS ion source.Scanning mass range was between m/z 100-1 350.Results: Thirty-nine chemical compounds in Ginseng were identified unequivocally.Conclusion: Chromatographic demonstration of 39 chemical compounds of Ginseng in one run is achieved by HPLC-TOF/MS,which provides a foundation for further study on the metabolism and action mechanism of traditional Chinese herbal medicine.
【Fund】: 国家“十一五”科技支撑计划课题(2006BAI08B03-07)~~
【CateGory Index】: R284.1
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