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《Academic Journal of Second Military Medical University》 2010-03
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Role of p38 MAPK signaling in lung injury following intestinal ischemia/reperfusion in mice

ZHENG De-yi1,2,WANG Jian-ming1,2,JIA Yi-tao1,FU Jin-feng 2,Lü Kai-yang1,LIAO Geng-jin1,2,ZHENG Xing-feng1,XIA Zhao-fan11.Department of Burns,Changhai Hospital,Second Military Medical University,Shanghai 200433,China2.Department of Burns,The Second Affiliated Hospital of Kunming Medical College,Kunming 650101,Yunnan,China  
Objective To investigate the possible role of p38 mitogen-activated protein kinase(MAPK) in lung injury following intestinal ischemia reperfusion(II/R) in mice.Methods Intestinal ischemia/reperfusion was induced by occluding the superior mesenteric artery for 45 min followed by 6 h reperfusion.C57BL/6 mice were randomly divided into sham-operated group(sham group),II/R group and II/R plus SB239063 treatment(SB239063 group),n=6/group.SB239063(3 mg/kg),a novel second-generation p38 MAPK inhibitor,was administered intraperitoneally one hour before clamping.Pulmonary p38 MAPK and phospho-p38 MAPK protein were measured by Western blotting analysis.Gene expression of TNF-α and IL-1β in the lung was analyzed by RT-PCR.The lung pathology was observed by optical microscope.Results Compared with the sham-operated group,pulmonary p38 MAPK activation was significantly increased 6 h after II/R(P0.01),whereas SB239063 could markedly attenuate p38 MAPK activation in lung tissue(P0.05).In addition,the increased TNF-α and IL-1β mRNA levels induced by II/R in lungs were significantly blocked by inhibiting p38 MAPK activation(P0.05).SB239063 treatment ameliorated the pathologic lung injury induced by II/R.Conclusion p38 MAPK plays an important role in lung injury induced by intestinal ischemia reperfusion(II/R) in mice,and inhibition of p38 MAPK activation prevents lung injury following II/R in mice.
【Fund】: 国家自然科学基金重点项目(30730091);; 上海市重点课题(08411952800)~~
【CateGory Index】: R574;R563.8
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