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《ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY》 1996-02
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Effect of Phytolacca acinosa polysaccharides I on DNA polymeraseα activity in murine splenocytes

Wang Hongbin;Wang Jin;Zheng Qinyue;Li Tiejun;Li Shutong;Chen Haisheng(Department of Pharmacology, College of Pharmacy,Second Military Medical University,Shanghai,200433)  
The purpose of the present study was to investigate the effect of Phytolacca acinosa polysaccharides(PAP-I) on DNA polymerase a activity of murine splenocytes and their proliferation.The activity of DNA polymerase a was measured by the method of [3H]-dTTP uptake by template DNA which was heated at 100℃for 5 min.The proliferation ability of murine splenocytes was tested by [3H]-TdR uptake by the splenocytes.The DNA polymerase a activity was found to be augmented by PAP-I at the dosage of 200μg/ml.In the same time,DNA synthesis of murine splenocytes was also increased. The DNA polymerase a activity from murine splencoytes,which were treated with ip PAP-I 5,10,50 mg/kg,was also measured in the present study.The results showed that PAP-I,10 mg/kg ip could significantly augment DNA polymerase a activity from murine splenocytes when the murine splenocytes were treated with Con A 5 μg/ml.DNA synthesis of murine splenocytes was also augmented by administration of PAP-I,10 mg/kg ip.These data indicate that enhancing effect of PAP-I on lymphocyte proliferation might be related with its augmenting effect on DNA polymerase a activity from murine splenocytes.
【CateGory Index】: R285.5
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