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《ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY》 1998-02
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A study of a novel TNFα mutant TNF60

Zhou Kai, Lou Yonghua,Jiao Binghua (Department of Microbiology, Department of Basic Medicine, Second Military Medical University, Shanghai, 200433)  
Objective: To investigate TNFα mutant with a high cytotoxicity or agonistic/antagonistic activity to NTFα. Methods and Results: Amino terminal deletion mutant of TNFα was prepared by protein engineering techniques. Encoding gene of mutant TNF60 with deletion of 59 amino acids at Nterminal of TNFα was constructed by PCR, which was cloned into pBV220 vector, and expressed in a proteasedefective E.coli SG1117. The recombinant protein accumulated as inclusion bodies was denatured, refolded and purified. Mutant TNF60 exhibited an enhancement of rhTNFα cytotoxicity against some cell lines such as L929, Hep2 and U937, though it had no cytotoxic activity itself. Conclusion: Mutant TNF60 has an augment of rhTNFα cytotoxic activity.
【CateGory Index】: R735.51
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【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Liu Li;Song Lihua;Liu Ji(Department of Hygienic Toxicology,School of Preventive Medicine);Cloning,expression,purification and biological activity analysis of TNF[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1996-05
2 Miao Weimin, Chen Jianhe, Wang Lianhua, Zhou Bingrong, Lou Yonghua, Jiao Binghua (Department of Microbiology, Department of Basic Medicine, Second Military Medical University, Shanghai, 200433);The amino acid sequencing on mutation region of recombinant human tumor necrosis factor α derivative 3a[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1997-04
3 LI Qing-yan~(1,2),SHI Ding-ji~(2,4*),CHEN Cui-li~(3,4),ZHAO Xing-gui~2, DENG Yuan-gao~2,ZHANG Yue-nan~2,LU Jin-yin~1 (1 College of Life Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 College of Marine Science and Engineering,Tianjin University of Sciences and Technology,Tianjin 300457,China;3 College of Arts & Science of Union University,Beijing 100083,China;4 Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China);Cloning of the Gene Encoding the Recombinant Human Granulocyte Colony-Stimulating Factor in Anabaena[J];Acta Botanica Boreali-occidentalia Sinica;2005-07
4 Yang Hui 1, Zhang Yingqi 1, Li Fangmei 1, Wang Junlou 1, Zhao Ning 1, Zhu Baoe 1, Yan Zhen 1, Su Chengzi 2 1Biotechnology Center, 2Department of Biochemistry, Fourth Military Medical University, Xi'an 710033;Purification of the new type human tumor necrosis factor expressed in E. coli[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1997-03
5 Lin Lizhu; Chen Chunmei; Yu Hong; Lu Qun; Chen Xiaolong;Yao Xihui; Chen Haiming; Zhao Shouyuan(Institute of Genetics);rhTNF-αpurification by colunm chromatography and its anti-tumor effects[J];;1994-05
6 KANG Rui juan 1, CAI Zhao ling 1, SHI Ding ji 2 (1.State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China; 2.Institute of Botany, Chinese Academy of Sciences, Beij;AN AIRLIFT PHOTOBIOREACTOR FOR MICROALGAE CULTIVATION[J];Chemical Reaction Engineering and Technology;2001-01
7 Zhou Xiaowei, Cheng Dusheng, Xu Xiuying, Huang Peitang Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071;High expression of hTNF α and its mutant protein in Escherichia coli[J];BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES;1996-03
8 ZHANG Chen, LIU Zhi-wei,GUO Yong (Department of Biology,Jiaying University, Meizhou 514015,China; College of Food Engineering and Biotechnology,South China University of Technology, Guangzhou 510640, China);Research Progress of Tumor Necrosis Factor and Its Genetic Engineering[J];Journal of Jiaying University;2002-06
9 Liu Li,Zhao Jianzeng, Xie Baoshu, Leng Aijun, Zhan Zhi (Dept. of Mole. Biology of Jiao Yu Tai Hospital, Beijing 100036);IN VIVO ANTITUMOR EFFECTS ON rhTNFα D11a TO S180 SARCOMA[J];IMMUNOLOGICAL JOURNAL;1997-02
10 Xu Xianxiu; Qiu Qifeng; Wang Ping; Dong Xueyin; Ding Yi(Department of Biochemistry, Nanjing University, 210093, Nanjing, PRC);A ONE-STEP PROCEDURE FOR PURIFICATION OF RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-ALPHA BY IMMUNOAFFINITY CHROMATOGRAPHY[J];JOURNAL OF NAIJING UNIVERSITY (NATURAL SCIENCES);1995-01
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LI Dan1,HAN Rui1,LI Xuan1,2,SHI Ding-ji1,3,ZHANG Peng-peng3,SONG Dong-hui1,WANG Xue-kui1(1.School of Marine Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457;2.School of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457;Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China);Optimization for Cultivation Conditions of Transgenic Escherichia coli Harbering TNF-α Gene[J];Biotechnology;2008-02
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