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《Endemic Diseases Bulletin (China)》 2002-02
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Establishment and Primary Application of Fast Dot-immunogold Staining for Detection of Antibodies to Cysticercosis

ZHENG Kui yang 1, DU Wen ping 1, LIU Yi sheng 1, ZHENG Xia 1, FU Lin lin 1, WU Zhong xing 2 (1. Xuzhou Medical College, Xuzhou, Jiangsu 221002, China; 2.Institute of Parasitic Diseases of Jiangsu Province, Wuxi, Jiangsu 214064,China)  
To set up a simple, economical and fast immunoassay for detection of antibodies in the patients with cysticercosis,the cysticercus cyst fluid antigens of Taenia solium were added onto mixed cellulose ester micropore filter membrane, then the serum antibodies against T. solium were detected by the fast dot immunogold staining assay (F Dot IGS). All of the 40 confirmed cases of cysticercosis showed positive reaction to the F Dot IGS. 40 healthy controls, 10 cases of clonorchiasis, 10 schistosomiasis and 10 paragonimiasis, all showed negative reactions but cross reaction obviously occurred with the sera of the 10 echinococcosis. The primary result showed that the F Dot IGS would be a fast, simpler and more economical assay with higher sensitivity and specificity, available for application in field situations and rapid diagnosis.
【CateGory Index】: R446.6
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