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《Endemic Diseases Bulletin》 2009-01
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Application of Yersinia pestis Label Gene of Plasmid pYC in Detecting Animals and Fleas

ZHANG Hong-ying, DONG Xing-qi, GUO Ying, WU Ming-shou (Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan 671000,China)  
Objective To observe the specificity and application of the label gene of Yersinia pestis plasmid pYC in surveillance of plague. Methods With primers yd1, yd2 and comparing primers fra and pla from the specific genes of Yersinia pestis plasmids, the infectious samples from testing and spot were detected by PCR. Results Eight infectious samples by testing all were positive. There were 6 positive samples in 22 samples collected from spot with the positive rate of 27.27%. There were 13 positive samples in 67 infectious fleas, the positive rate was 19.40%. The specificity and sensitivity were consistent about testing primers and comparing primers. Conclusions Combining the primers yd1, yd2 and pla, fla can establish a rapid PCR method of plague. It will be of practical importance in diagnosis and surveillance of plague in Yunnan, Guangxi and Guizhou provinces.
【Fund】: 国家自然科学基金资助项目(39960006);; 云南省中青年学术和技术带头人培养经费资助项目(1999C0026G)
【CateGory Index】: R378.61
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