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《ENDEMIC DISEASES BULLETIN (CHINA)》 1996-03
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Analysis of Specific Antigens Extracted from Excretory Secretory Products of Trichinella spiralis Muscle Larvae

Li Liao;Lei Li;Feng Ruiyuan;Yan Heping(Teaching and Research Section of Parasitology,West China University of Medical Sciences,Chengdu 610044)  
Muscle larvae were obtained from infected mice by peptic digestion. The larvae were culturedin RPMI1640 medium at 37℃. The medium was changed every 24 hours for 10 days in order to remove thedead larvae Trichinella spiralis,and the rate of dead larvae must be no more than 5%。The collected super-natant,excretory-secretory antigen:ESA1d,ESA26,ESA46,ESA6d,ESA8d and ESA10d were analysed bySDS-PAGE with silver staining,Results showed that the stained bands of these kinds of ESA were similar innumber and site. The molecular weight of these banks were between 14~96 kD with three chief bands in 48,53 and 58 kD.ESA1d,ESA2d,ESA5d,ESA8d and ESA10d were analysed by Western-blot and the results showedthat the effect of immune recognition were similar.The sera from patients with trichinosis were recognizedwith 7~8 antigenic bands in chief bands of 46,48,50/53 and 58 kD. The sera from health persons and pa-tients with ascariasis or cysticerosis showed no any bands The sera from patients with paragonimiasis wererecognized with 2~3 antigenic bands at 14~24 kD,and those from patients with schistosomiasis with 2~3antigenic bands at 14~18 kD. These results indicate that the proteins of ESA between 46~58 kD were highlyspecific antigens.
【Fund】: 卫生部基金
【CateGory Index】: R383.15
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