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《Chinese Journal of Medicinal Guide》 2001-03
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The Improvement of the Method of Neonatal Rat Myocytes Culture

Shen Jing, Xie Miao-rong , Xu Yong, Lu Yan, Shen Lu - hua (Beijing Friendship Hospital affiliated to the Capital University of Medical Science 100050)  
Objective: to improve the method of neonatal rat myocytes culture. Methods: The techniques used in our new method were based on Simpson's, and they were compared with each other. Results: From the new method, the dissociation yielded an average 1.6×106 cells per heart. The ratio of trypsin blue-negative cells were 76.08% , 93.32% and 91.89% on immediately and 5, 8 days after digestion respectively, The ratio of beat cells spontaneously were 61.63% and 90.69 % on 5 and 8 days respec-tively after the beginning of cell culture. All parameters above increased in our new method than Simpson's. Conclusion: our new improved method is better at getting more free and living myocytes from neonatal rat.
【CateGory Index】: R-332
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