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Determination of Fat-soluble Constituents in Cordyceps Militaris Fruitbodies Using GC-MC

Cai Qi-hui1,~2,Tian Hong-xianl,~(2*),Liu Zhu-xian1,et al. (~1Jishou University of biological resources and the environment Academy of Sciences Hunan 41600,China;~2Forest product chemical industry key laboratory natural medicine Research center of Hunan Jishou Hunan 416000,china)  
Objective:To investigate the fat-soluble constituents in Cordyceps militaris Fruitbodies with GC-MS method.Methods:To take the fresh Cordyceps militaris Fruitbodies,using low-temperature drying,grinding.Then extracted by petroleum ether or ether in refluxing and esteried,and inally was determined using GC-MC.Results:Two kinds of fat-soluble constituents in petroleum ether extract and ether extract are the same,Cordyceps militaris fruitbodies of ether to detect substanoes-23,identified 17 kinds of fat-soluble components,mainly including octadecadienoic acid,octadecenoic acid,hexadecanoic acid,octadecanoic acid,dehydroergoergosterol,2,6,10,14,18,22-tetracosahexsene the relative percentage of 37.78%,35.42%,6.67%,9.57%,3.33%,1.99%.Conclusion:The method is simple,fast,high sensitivity,as Cordyceps militaris Fruitbodies Identification on the quality.
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